Separation and Enrichment of Casein Phosphopeptides by SiO2@TiO2

Abstract

Abstract: Titania has the characteristics of selective adsorption of phosphopeptides. In order to obtain TiO2 with high specific surface area and improve the separation and enrichment effect of phosphopeptide, SiO2@TiO2 composite materials were prepared by layer-by-layer self-assembly method. The specific surface area and average pore diameter of SiO2@TiO2 are 116.26 m2/g and 8.42 nm, respectively. The core-shell composite materials were used in the separation and enrichment of casein phosphopeptides (CPPs). The adsorption rate of CPPs determined by high performance liquid chromatography (HPLC) is 93.0%, and the desorption rate is 81.5%. The sequence of the five phosphopeptides in the eluent was identified by Q-TOF LC/MS secondary mass spectrometry, PS*H, PS*PR, S*SRS*E, PHS* and PS*SRS*EP, respectively. Theophylline is the internal standard of CPPs. According to the analysis results of HPLC, the range of recoveries of the samples of CPPS, calcium tablets and infant formula is 92.5%-95.1%. It can be concluded that the properties of SiO2@TiO2 core-shell composite are excellent, the effects of the separation and enrichment of CPPs are significant, and the proposed method is very accurate and can be used to determine CPPs in real samples.

Key words: SiO2@TiO2 composite materials, solid phase extraction, separation and enrichment, casein phosphate peptides (CPPs)

CLC Number:

中图分类号：TS202.3

Zi-Tao Jiang. Separation and Enrichment of Casein Phosphopeptides by SiO2@TiO2[J]. FOOD SCIENCE, 0, (): 0-0.

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