Study on Protective Effect of Resveratrol on Nerve Cells of Aging Mice and Its Mechanism

Abstract

Abstract: Objective: To investigate the protective effect and mechanism of resveratrol on nerve cells in aging mice induced by D-galactose (D-gal). Methods: The aging model of mice was established by intraperitoneal injection of D-gal. The change of body weight and brain organ index were calculated. Apoptosis rates, reactive oxygen species levels and mitochondrial membrane potential in cerebral cortical neurons were measured by flow cytometry. Results: From the aspect of cell viability, compared with the normal group, the apoptotic of the neurons in the model group was significantly increased (P<0.01), indicating that D-gal significantly damages the nerve cells in mice. Compared with the model group, the apoptotic rates of c nerve cells in the three resveratrol groups were significantly lower (P<0.01), indicating that resveratrol has protective effects on D-gal-induced aging mice neural cells. Further results showed that compared with the normal group, in the model group the ROS content in nerve cells increased significantly (P<0.01), and the Mitochondrial Membrane Potential significantly decreased (P<0.01). The mitochondrial Cyt c content decreased significantly (P<0.05), cytoplasmic Cyt c content increased significantly (P<0.05), mitochondrial Caspase-9 and Caspase-3 activity increased significantly (P<0.01), indicating that D-gal leaded to mitochondria cause damage. Compared with the model group, the amount of ROS in the nerve cells of three RSV groups significantly decreased (P<0.01); MMP significantly increased (P<0.01); Cytochrome c content of mitochondria significantly increased, while cytoplasm Cyt c was significantly decreased (P<0.05); the activities of Caspase-9 and Caspase-3 were significantly decreased (P<0.01), indicating that resveratrol had a protective effect on nerve cells of aging mice through the mitochondrial pathway. Conclusion: Resveratrol can protect nerve cells from aging mice induced by D-gal through mitochondrial mechanisms.

Key words: resveratrol, D-galactose, apoptosis, neuroprotection, mitochondrial mechanism

CLC Number:

TS201.4

Xian-Yi ZHANG LI LU wen-juan li. Study on Protective Effect of Resveratrol on Nerve Cells of Aging Mice and Its Mechanism[J]. FOOD SCIENCE, 0, (): 0-0.

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