Identification of heterologous species in donkey-hide gelatin based on specific peptides

Abstract

Abstract: Donkey-hide gelatin is a valuable glue medicine. In recent years, due to the scarcity of donkey skin materials, there is a serious phenomenon that adulteration in gelatin and the incorporation of other animal skin glue. Common methods of adulteration include: the low-grade rubber materials such as bovine-hide gelatin and pig-hide gelatin are incorporated into the gelatin, and the horse skin is mixed in the raw material. In order to identify other animal glue in donkey-hide gelatin, this study combined with bottom-up proteomics techniques to find and identify thermostable characteristic peptides from different species. First of all, we found the characteristic peptides of three kinds of animal glue medicine (donkey-hide gelatin, pig-hide gelatin and bovine-hide gelatin). At the same time, we found that thermostable specific peptides containing no hydroxyproline in three species are located at similar positions in the alpha 1 sequence of collagen I in the type I collagen alpha 1 sequence. By investigating the sequence characteristics of collagen-derived species-specific peptides, we speculated the cleavage rule of collagen during the Industrial processing. Secondly, two horse skin characteristic peptides, derived from keratin and unnamed protein of horse, were obtained for the identification of horse hide glue in donkey-hide gelatin. This study can not only realize the identification of donkey-hide gelatin and heterogeneous animal skin glue, but also provide technical support for the authenticity identification of donkey-hide gelatin and the discovery of of more collagen source specific peptides.

Key words: donkey-hide gelatin, proteomics, specific peptide, multiple reaction monitoring, mass-spectrometric technique

CLC Number:

TS201.6

Fang Fang Jiu-Kai ZHANG Xue-Ting MA ying chen. Identification of heterologous species in donkey-hide gelatin based on specific peptides[J]. FOOD SCIENCE, 0, (): 0-0.

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