Abstract Purpose: In order to get the mechanism of limonin, nomilin and naringin on Hela cell proliferation and apoptosis, the white sponge layer and seeds of the Gaotai honey pomelo were as raw materials, the extraction, purification and detection of three bitter substances were carried out respectively. Methods: Three bitter substances were extracted by ultrasonic assisted organic solvent extraction, purified by multiple recrystallization, and detected by UPLC. CCK8, cell scratches and RT-PCR were used to verify the anti-cancer effect from three bitter substances. Results and conclusions: A method for the simple, rapid and accurate determination of limonin, nomilin and naringin in Gaotai honey pomelo by ultra performance liquid chromatography（UPLC） was developed. The results of bioactivity test confirmed that the inhibition of Hela cell growth by three bitter substances was closely related to the inhibition of cell proliferation and migration.
Received: 08 October 2018
Published: 25 October 2019