FOOD SCIENCE ›› 0, Vol. ›› Issue (): 0-0.

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Determination of 9 kinds of Macrolides Antibiotics in Pork Samples by Ultra Performance Liquid Chromatography-Quadrupole Obitrap High Resolution Mass Spectrometry

2, 2,Zhuo LIU   

  • Received:2018-10-26 Revised:2019-11-29 Online:2020-02-25 Published:2020-03-02

Abstract: A rapid method was presented for the determination of 9 macrolides antibiotics in pork sample by ultra-performance liquid chromatography-quadrupole orbitrap high resolution mass spectrometry. Pork samples were extracted by acetonitrile and cleaned with n-hexane, then separated by Waters Acquity BEH C18 column(100 mm×2.1 mm, 1.7 μm) using a binary solvent system composed of methanol and water(0.1% formic acid) by gradient elution. The analytes were determined by quadrupole-orbitrap high resolution mass spectrometry in full-MS and data-dependent scan (ddMS2) mode. The results showed that mass accuracy error of the 9 antibiotics were lower than 1.0×10-6,linear relationships is well in the range of 0.5-100 ng/mL for 9 antibiotics, and correlation coefficients were higher than 0.998. The LOD is in the range of 0.05-0.2 μg/kg and the LOQ is in the range of 0.1-0.4 μg/kg. Recovery of the method is in range of 69.4%-107.6% with spike levels of 0.1-4.0μg/kg, relative standard deviations were lower than 10%. The method is simple, rapid, accurate, which is suitable for the rapid screening and quantitative analysis of 9 kinds of macrolides antibiotics in pork sample.

Key words: ultra high performance liquid chromatography, quadrupole/orbitrap high resolution mass spectrometry, pork samples, macrolides antibiotics, Determination

CLC Number: