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Effect of Ferulic Acid on the Mechanism of Tyramine Production by Enterococcus faecalis and Enterococcus faecium

Lin-Lin XUE Bin-Bin LI 2   

  • Received:2018-11-09 Revised:2019-09-23 Online:2019-11-25 Published:2019-12-02

Abstract: This study detected the effects of ferulic acids on the growth, gene expression and tyramine accumulation by E. faecalis XL-M66 and E. faecium XL-M76. Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) was used to analyze the related gene expression of Tyrosine decarboxylase (TDC) locus in E. faecalis XL-M66 and E. faecium XL-M76. Additionally,the accumulation of tyramine during the 48h culture of two Enterococcus strains was monitored by High Performance Liquid Chromatography (HPLC). Results showed that ferulic acid had no effect on the transcription of the tyrDC and tyrP genes (P>0.05) when no tyrosine substrate was added, but promotes transcription of the tyrS gene (P<0.05). On the contrary, with the addition of tyrosine, ferulic acid had no effect on the expression of tyrS gene (P>0.05), but it could significantly (P<0.05) inhibit the expression of tyrDC and tyrP genes. Moreover, Ferulic acid significantly (P<0.05) inhibited the growth of E. faecalis XL-M66 and E. faecium XL-M76 and reduced tyramine production by 27.0% and 19.9%, respectively.

Key words: ferulic acids, Enterococcus faecalis, Enterococcus faecium, tyramine, tyrosine decarboxylase, Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR)

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