FOOD SCIENCE ›› 0, Vol. ›› Issue (): 0-0.

• Safety Detection •     Next Articles

Simultaneous Determination of Pinoxaden and Clodinafop-propargyl and Cloquintocet-mexyl in Barley by High Performance Liquid Chromatography Tandem Mass Spectrometry

  

  • Received:2018-11-06 Revised:2019-12-20 Online:2020-03-25 Published:2020-03-23

Abstract: Abstract: A method for simultaneous determination of two herbicides, pinoxaden, Clodinafop-propargyl and its safety agent Cloquintocet-mexyl in barely?by high performance liquid chromatography-tandem mass spectrometry with QuEChERS. The samples was extracted -by acetonitrile, cleaned up with C18 and PSA . The analytes were separated by a reversed phase C18 column(XBridge-C18 column) , and gradiently eluted with 0.1% formic acid solution and acetonitrile as mobile phase.? The samples?were?analyzed under the multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI+) and quantified?by external standard with matrix matched method. The good linearity was obtained from pinoxaden, Clodinafop-?propargyl and Cloquintocet-mexyl in barely?at the concentration of 0.1-100 μ g / L, and the linear correlation coefficient was higher than 0.997. Respectively, the limit of detection (LOD) of the method was 0.05, 0.02 μg/L and 0.01 μg/ L. The pinoxaden average recoveries of the three concentration levels were 76.87%- 80.722%, the Clodinafop-propargyl average recoveries of the three concentration levels were 95.07%-99.15%, and the Cloquintocet -mexyl average recoveries of the three concentration levels were 96.73%- 103.55%. The repeatability expressed as relative standard deviations (RSD) was ranged from 2.4% to 9.2%(n = 5).?The method was used to evaluate the residue of the above three compounds in local market barley grain and seedling powder. And the method provides technical support for the determination of pinoxaden residues in barley products.

Key words: Keywords: Barley, pinoxaden, clodinafop-propargyl, cloquintocet-mexyl, high performance liquid chromatography -tandem mass spectrometry

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