The inhibitory regulation effect of phycocyanin on non-small cell lung cancer cells based on RNA-seq

Abstract

Abstract: Phycocyanin (PC) is a natural food colorant used in kinds of food industries in China. It is also a type of natural functional edible protein which possesses good anti-tumor ability. Our present study showed that phycocyanin could significantly inhibit proliferation and induce apoptosis of non-small cell lung cancer (NSCLC) H460 cells. The high-throughput RNA-seq analysis showed that the expression levels of 2532 genes were affected by phycocyanin, including 1491 up-regulated genes and 1041 down-regulated genes. GO analysis showed that these differentially expressed genes mainly participated in cell proliferation, cell apoptosis and cytokine response biological processes. Cluster analysis showed that proliferation-related genes like Cdk2, Cdc25, PCNA, TLR4 were down-regulated, p27 and p21 were up-regulated after phycocyanin treatment. Meanwhile, apoptosis-related genes like Bcl-2 and NKD1 were significantly down-regulated, CCT6 and GBP2 were significanltly up-regulated after phycocyanin treatment. KEGG pathway analysis showed that PI3K-Akt signaling was one of the important pathway of phycocyanin in H460 cells. The present study investigated the potential mechanism of phycocyanin in NSCLC H460 cells, which provided solid foundation for target treatment of lung cancer and application of functional foods.

Key words: phycocyanin (PC), non-small cell lung cancer (NSCLC), H460 cells, RNA-seq, cell proliferation, cell apoptosis

CLC Number:

Shuai HAO. The inhibitory regulation effect of phycocyanin on non-small cell lung cancer cells based on RNA-seq[J]. FOOD SCIENCE, 0, (): 0-0.

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