FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (6): 325-330.doi: 10.7506/spkx1002-6630-20180111-131

• Safety Detection • Previous Articles     Next Articles

Comparison and Application of RNA Extraction Methods for Detection of Hepatitis A Virus in Food Samples

FENG Huawei1, YAN Pingping2, AI Haixin1,3, LI Sijia4, MA Lidan5, LIU Hongsheng1,3,*   

  1. 1. School of Life Sciences, Liaoning University, Shenyang 110036, China; 2. Danlian Entry-Exit Inspection and Quarantine Bureau, Dalian 116400, China; 3. Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning, Shenyang 110036, China; 4. Donggang Comprehensive Agricultural Law Enforcement Brigade, Dandong 118000, China; 5. Dandong Entry-Exit Inspection and Quarantine Bureau, Dandong 118000, China
  • Online:2019-03-25 Published:2019-04-02

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Abstract: Objective: Three RNA extraction kits were compared to select the best one for RNA extraction from hepatitis A virus (HAV) in foods, aiming to provide a technical basis for foodborne HAV RNA detection in laboratories. Methods: After artificial contamination of known negative samples, three commercial nucleic acid extraction kits, ABI AM1836, QIAGEN 74104 and ROCHE 11858882001, were used to extract HAV RNA and their performance was evaluated in terms of extraction efficiency, inhibitor removal efficiency and sensitivity, and the optimized extraction kit was applied to real samples. Results: ROCHE 11858882001 was the best extraction kit, followed by QIAGEN 74104 and ABI AM1836. Of 101 samples, one sample of blue honeysuckle was positive while the rest were negative. Conclusion: ROCHE 11858882001 is a rapid and effective method for extracting HAV RNA, and thus it is suitable for the routine detection of foodborne HAV.

Key words: hepatitis A virus, RNA extraction method, real-time fluorescence RT-PCR, food samples

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