FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (7): 228-237.doi: 10.7506/spkx1002-6630-20180913-120

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Pichia membranaefaciens Combined with Chitosan Treatment Induces Resistance to Anthracnose in Citrus Fruit

TIAN Ligang, PENG Ru, YAO Shixiang, DENG Lili, ZENG Kaifang   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, China
  • Online:2019-04-15 Published:2019-05-05

Abstract: This study was undertaken in order to investigate the combined effect of Pichia membranaefaciens and chitosan on controlling anthracnose and inducing anthracnose resistance in citrus fruit (Citrus sinensis L. Osbeck. cv. Jincheng 447) inoculated with Colletotrichum gloeosporioides. The results showed that the application of P. membranaefaciens (1 × 108 cells/mL) or chitosan (0.1 g/L) were effective in inhibiting anthracnose but inferior to their combination. The combined treatment increased the contents of some signal substances including NO, salicylic acid and H2O2 as well as the contents of intermediate metabolites of phenylpropane (ferulic acid, p-coumaric acid and chlorogenic acid), and induced higher activities of defense-related enzymes including phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, 4-coumarate:coenzyme A ligase, peroxidase and polyphenoloxidase and higher activities of pathogenesis-related proteins (PR-proteins) such as chintinase and β-1,3-glucanse. In conclusion, P. membranaefaciens combined with chitosan induces anthracnose resistance in citrus fruit.

Key words: Pichia membranaefaciens, chitosan, Colletotrichum gloeosporioides, induced resistance, citrus fruit

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