Abstract: ABSTRACT：To establish a method for the rapid detection of glyphosate(GLY) and glufosinate-ammonium(GLUF) in tea by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).After the extraction of NaOH solution, regulated pH value with HCl, the extract was purified with PSA ，and then precolum-derived with 9-fluorenylmethyl chlorformate ((FMOC-Cl)) in borate buffer.The positive ion multiple reaction monitoring (MRM) was measured and the external standard method was quantified. The standard curve for GLY and GLUF were linear over the concentration range of 0.005~0.5 μg/mL，correlation coefficient were more than 0.990.The limit of detection (LOD) and the limits of quantization(LOQ) were 0.05 mg/kg and 0.08 mg.kg.The samples spiked with 0.08, 0.1, 1, 2 and 4 mg/kg showed the recovery rate of GLY and GLUF were 75.6%~95.5% and 76.0%~96.6%, and the relative standard deviations (RSDs) ( n=10) of GLY and GLUF were 3.24%~8.38% and 3.05%~7.85%. This method can detect the GLY and GLUF residues in green tea, with a better precision, higher accuracy, better specificity, and the result is satisfied.

Key words: KEY WORDS：ultra-high performance liquid chromatography-tandem mass spectrometry, glyphosate, glufosinate-ammonium, residue, tea