Effect of Acid Stress on the Biosynthesis of S-Adenosylmethionine and Glutathione and Its Physiological Mechanism

Abstract

Abstract: Effect of different pH conditions on the biosynthesis of S-adenosylmethionine (SAM) and glutathione (GSH) by Candida utilis under high cell density culture were investigated. The moderate acid stress (pH 3.5) was found to favor the increase in SAM and GSH biosynthesis capability, and in turn improved the co-production of SAM and GSH by the yeast cells. Based on the assay of key enzymes, the determination of energy substances, and the measurement of intracellular redox indexes, the moderate acid stress was shown to increase the activities of SAM synthase, γ-glutamylcysteine synthase, hexokinase, ATP synthase and catalase, elevate intracellular levels and regeneration abilities of NADH and ATP, all of which provided sufficient supply of substrates and energy substances for the over-production of SAM and GSH. Results in this study presented adequate evidence to reveal the physiological mechanism underlying improved SAM and GSH biosynthesis by moderate acid stress, which also provided a feasible idea for the over-production of other similar small molecular active compounds.

Key words: Candida utilis, S-adenosylmethionine, glutathione, co-production, acid stress

CLC Number:

TS201.3

Da-Hui WANG Gong-Yuan WEI. Effect of Acid Stress on the Biosynthesis of S-Adenosylmethionine and Glutathione and Its Physiological Mechanism[J]. FOOD SCIENCE, 0, (): 0-0.

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