FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (11): 7-13.doi: 10.7506/spkx1002-6630-20180510-163

• Basic Research • Previous Articles     Next Articles

Inhibitory Effect of Sodium Fumarate on Quorum Sensing and Spoilage Capacity of Pseudomonas fluorescens

SUN Xiaojia1, LI Tingting2,*, HE Binbin1, MEI Yongchao1, WANG Dangfeng1, XIE Jing3, LI Jianrong1,*   

  1. 1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Laboratory of Liaoning Province, the Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, College of Food Science and Technology, Bohai University, Jinzhou 121013, China; 2. College of Life Science, Dalian Minzu University, Dalian 116600, China; 3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2019-06-15 Published:2019-06-28

Abstract: In this paper, Chromobacterium violaceum CV026 strain was used as a test model to investigate the effect of sodium fumarate on quorum sensing and spoilage capacity of Pseudomonas fluorescens. The inhibitory effect of sodium fumarate on biofilm formation was evaluated with a microplate reader and optical microscopy, and its effect on the secretion of signal molecules in Pseudomonas fluorescens was determined by gas chromatography-mass spectrometry (GC-MS). The results showed that sodium fumarate could significantly reduce the production of violacein in strain CV026 at sub-minimum inhibitory concentrations. Violacein production was inhibited 50.95% by sodium fumarate at 2.0 mg/mL while not affecting the growth of CV026. Sodium fumarate could effectively inhibit spoilage characteristics of Pseudomonas fluorescens as reflected by biofilm formation, swarming and swimming motility, and extracellular protease activity and this effect was positively correlated with its concentration. By GC-MS analysis, we also found that sodium fumarate significantly inhibited the secretion of the signal molecules N-acylhomoserine lactones (AHLs). Therefore, sodium fumarate has great inhibitory activity and can be developed as a quorum sensing inhibitor for refrigerated storage and preservation of aquatic products.

Key words: sodium fumarate, Pseudomonas fluorescens, quorum sensing, spoilage capacity, inhibitory effects

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