FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (12): 290-295.doi: 10.7506/spkx1002-6630-20180424-308

• Safety Detection • Previous Articles     Next Articles

Discrimination of Geographical Origins of Rape Honey by Polyphenol and Oligosaccharide Measurements Combined with Partial Least Square-Discrimination Analysis

SHE Seng1,2, LI Yi1,3, SONG Hongbo2, CHEN Lanzhen1,3,*   

  1. 1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. Laboratory of Quality and Safety Risk Assessment for Bee Products (Beijing), Ministry of Agriculture and Rural Affairs, Beijing 100093, China
  • Online:2019-06-25 Published:2019-06-28

Abstract: In this study, 6 kinds of oligosaccharides in rape honey were determined by gas chromatography-mass spectrometry and 18 kinds of phenols by high-performance liquid chromatography-mass spectrometry (HPLC-MS). A new method was used to discriminate rape honey samples from different geographical origins through analysis of oligosaccharides and polyphenols combined with partial least squares discriminant analysis (PLS-DA). For this study, we collected 51 samples from Zhongxiang city of Hubei province, Yancheng city of Jiangsu province and Gangcha county of Qinghai province, among which there are significant geographical, climatic and environmental differences. The results of analysis of variance (ANOVA) showed that there were significant differences in the turanose content of rape honey samples from the three regions, and the oligosaccharide content of rape honey form Gangcha was relatively higher. There were significant geographical differences in the contents of most polyphenols and the polyphenols content of rape honey from Zhongxiang was relatively higher. Multivariate statistical analysis showed that polyphenols were a significant indicator for the geographical origin of honey, and the geographical origins of rape honey could be predicted with an accuracy of 97% by the contents of oligosaccharides and polyphenols combined with PLS-DA.

Key words: oligosaccharides, polyphenols, rape honey, geographical traceability, OPLS-DA

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