Hepatoprotective Effect of Dendrobium Candidum Polysaccharides on Alcohol-induced Acute Liver Injury in Mice

Abstract

Abstract: Polysaccharides were isolated from Dendrobium Candidum by hot water extraction, and explored for hepatoprotective effect on alcohol-induced acute liver injury mice. Mice were intragastrically infused with 50% of alcohol to establish alcohol-induced acute liver injury mouse models. At the end of the experiment, blood was taken by removalling eyeball, and then the mice were sacrificed with cervical dislocation to take their liver tissue. Red blood cell (RBC), haematocrit (HCT), mean corpuscular (MCV), white blood cell (WBC) and hemoglobin (HGB) were measured through blood cell counter. Superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) and triglyceride (TG) content in liver, as well as alanine transaminase (ALT), aspartate transaminase (AST) and triglyceride (TG) levels in serum were measured. Histopathological changes in liver tissues were examined to evaluate the hepatoprotective effect of Dendrobium Candidum. The results showed that the levels of RBC, WBC in blood, ALT, AST, TG in serum, and SOD activity, GSH-Px and TG content in liver, as well as liver index had significant difference (P<0.05 or P<0.01) between control group and model group. Dendrobium Candidum exhibited an obvious reduction in liver index, RBC, WBC, ALT, AST and TG levels (P<0.05 or P<0.01) in mice suffering from alcohol-induced acute liver injure. The activities of SOD and GSH-Px in liver were enhanced (P<0.05 or P<0.01), and promoted the recovery of hepatic tissue injury. Dendrobium Candidum polysaccharides had significantly hepatoprotective effect on alcohol-induced acute liver injury mice.

Key words: Dendrobium Candidum, alcohol, acute liver injury, hepatoprotective

CLC Number:

R285.5

Shen XianRong jiang dingwen. Hepatoprotective Effect of Dendrobium Candidum Polysaccharides on Alcohol-induced Acute Liver Injury in Mice[J]. FOOD SCIENCE, 0, (): 0-0.

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