FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (13): 187-194.doi: 10.7506/spkx1002-6630-20180620-388

• Nutrition & Hygiene • Previous Articles     Next Articles

Soluble Soybean Polysaccharides Ameliorate L-Carnitine-Induced First-Pass Metabolism in the Small Intestine of Mice

LI Wenfeng, TAO Wen, CHEN Luhong, ZHENG Qiaoran, ZHOU Feng, TAN Si, XING Jie   

  1. School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408000, China
  • Online:2019-07-15 Published:2019-07-23

Abstract: Objective: To study the effect of soluble soybean polysaccharides (SSPS) on intestinal metabolism, oxidative stress and inflammation-related proteins in mice fed L-carnitine. Methods: A total of 24 male Kunming mice were randomly divided into three groups: normal group, L-carnitine group and SSPS group. At the end of the 56-day feeding period, all mice were sacrificed to assess the changes in the levels of P-glycoprotein (P-gp), multidrug resistance protein (MRP) 1, MRP3, interleukin (IL)-1, IL-6, tumor necrosis factor-α (TNF-α), uridine diphosphateglucuronosyltransferase (UGT), sulfotransferase (SULT), and malondialdehyde (MDA) in the small intestine. Results: The concentrations of P-gp and MRP3 in the small intestine homogenate supernatant of mice in the L-carnitine group were significantly higher than that of the normal group (P < 0.05). However, SSPS significantly decreased P-gp concentration (P < 0.05), and slightly decreased MRP3 concentration in the small intestine of L-carnitine-fed mice, suggesting that SSPS could inhibit L-carnitine-induced first pass metabolism. Additionally, co-treatment of SSPS and L-carnitine on mice effectively prevented the increase of MDA content, the decrease of superoxide dismutase (SOD) activity, and the weakening of hydroxyl radical-scavenging capacity, indicating that SSPS could inhibit L-carnitine-induced oxidative stress. However, the activities of SULT and UGT, and the concentrations of IL-1, IL-6 and TNF-α in the small intestine did not differ significantly among all groups of mice (P > 0.05), suggesting that excessive L-carnitine and SSPS treatment did not affect the II phase metabolism or lead to inflammation. Furthermore, metabonomic analysis showed that SSPS could inhibit the disorders of energy metabolism and lipid metabolism caused by long-term excessive intake of L-carnitine. Conclusion: The first pass metabolism induced by L-carnitine can be effectively prevented by SSPS via controlling oxidative stress and metabolic disorders.

Key words: soluble soybean polysaccharide, L-carnitine, first pass metabolism, oxidative stress, inflammation

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