Abstract: In order to reveal the diversity and community structure changes of Xiangyang kohlrabi during the fermentation, Illumina Miseq high-throughput sequencing technology was used. A total of 4 phyla, 19 classes, 56 orders, 106 families, 190 genera, 303 species and 516 OTUs were identified in the 24 samples. Both cluster analysis and principal coordinate analysis showed that the 24 samples could be divided into three clusters based on structural characteristics of fungal community, i.e. early-fermentation, middle-fermentation and later-fermentation. The total number of common OTU in the three fermentation stages was 124. After the removal of unclassified fungi, the dominant phylum in all samples was Ascomycota, with a relative abundance of 74.2% to 99.8%, followed by Basidiomycota. At the level of order, Xylariales was the dominant fungal at the early fermentation, with a relative abundance of 45.7% to 60.2%. In the subsequent fermentation process, Saccharomycetales replaced Xylariales and took an absolute advantage, with a relative abundance of 63.6% to 99.5%. At the level of genus, Monographella was the dominant fungus in the early fermentation, and Debaryomyces and Zygosaccharomyces were the dominant fungal in the middle and late fermentation.

Key words: kohlrabi, Fungal diversity, High-throughput sequencing