Determination of Flavonoids in the Supercritical CO2 Extract of Sea Buckthorn Fruit by Using Ultra-High Performance Liquid Chromatography-Mass Spectrometry

doi:10.7506/spkx1002-6630-20181015-138

FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (18): 273-280.doi: 10.7506/spkx1002-6630-20181015-138

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Determination of Flavonoids in the Supercritical CO2 Extract of Sea Buckthorn Fruit by Using Ultra-High Performance Liquid Chromatography-Mass Spectrometry

DING Lina, QIU Yiyi, SHU Tong, RUAN Hui

(1. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; 2. Qinghai Food Inspection and Testing Institute, Xining 810008, China; 3. Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, China; 4. Ningbo Research Institute, Zhejiang University, Ningbo 315100, China)

Online:2019-09-25 Published:2019-09-23

Abstract

Abstract: Sea buckthorn fruit oil was obtained by supercritical CO2 extraction, and its flavonoid components were analyzed by ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) through Peakview workstation matching followed by tandem mass spectrometric analysis and comparison of ultraviolet spectroscopic data and mass spectral data with those of reference standards as well as with literature data. A total of 18 flavonoid compounds were determined, including eight flavonol aglycones and flavonol glycosides, three dihydroflavonol aglycones and dihydroflavonol glycosides, five flavonoid aglycones and flavonoid glycosides, one dihydroflavonoid aglycon and one flavanol glycoside. To our knowledge, apigenin-6-C-glucoside-8-C-xyloside, catechin-7-glucopyranoside, alizarin, violet and locustin were found in sea buckthorn for the first time.

Key words: sea buckthorn, flavonoid, supercritical CO2 extraction, ultra-high performance liquid chromatography-mass spectrometry

CLC Number:

TS201.2

DING Lina, QIU Yiyi, SHU Tong, RUAN Hui. Determination of Flavonoids in the Supercritical CO2 Extract of Sea Buckthorn Fruit by Using Ultra-High Performance Liquid Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2019, 40(18): 273-280.

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Determination of Flavonoids in the Supercritical CO2 Extract of Sea Buckthorn Fruit by Using Ultra-High Performance Liquid Chromatography-Mass Spectrometry

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