FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (18): 335-341.doi: 10.7506/spkx1002-6630-20181031-372

• Safety Detection • Previous Articles    

Quantum Dot-Based Assay for Rapid Simultaneous Detection of Lead and Cadmium

LIU Beibei , CAO Lin   

  1. (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:2019-09-25 Revised:2019-09-25 Online:2019-09-25 Published:2019-09-23

Abstract: The objective of this study was to establish a quantum dot-based fluorescence immunochromatographic assay for the rapid simultaneous detection of lead and cadmium. Mouse anti-Cd2+-IEDTA and anti-Pb2+-IEDTA monoclonal antibodies were separately coupled with CdSe/CdS core-shell quantum dots embedded in polystyrene microspheres. The optimum reaction time of the dual test strip was 5 min. Pb2+ concentrations of 1–500 ng/mL, and Cd2+ concentrations of 1–400 ng/mL were detectable using the proposed method with coefficients of variation of less than 10%. Moreover, there was no cross-reactivity with other metal ions. The results of this assay were correlated well with those of inductively coupled plasma-mass spectrometry (ICP-MS) for commercial meat samples. This dual fluorescence immunochromatographic assay provided an applicable method for the rapid and simultaneous quantitative detection of lead and cadmium in meat.

Key words: cadmium, lead, monoclonal antibody, quantum dots, dual assay

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