Comparison on the Physicochemical and Biochemical Characteristics of Crude Polysaccharides from Longan Pulps Dried by Different Methods

Abstract

Abstract: Crude polysaccharides (i.e., FLP, HDLP, VDLP, IDLP and FDLP) were extracted from fresh and hot air, vacuum, infrared and freeze-dried longan pulp. Their basic composition, FTIR spectral characteristics and molecular mass distribution were analyzed to evaluate their antioxidant activity and immune stimulation activity, and their stability in simulated digestive system was investigated in this study. Five kinds of crude polysaccharides mainly contained glucans and proteins with different mass fractions, and amino acid compositions were obviously different. In addition to the polysaccharide and protein (amide) infrared spectrum characteristic absorption, FLP, HDLP, VDLP, IDLP and FDLP there were significant differences in the molecular weight distribution, the ratio of the average molecular weight of 51.37:28.33:5.95:7.86:1.00. Comprehensive comparison showed that FDLP had stronger scavenging ability of DPPH free radicals and hydroxyl free radicals, while HDLP had stronger ability to stimulate the generation of NO and tnf-alpha in macrophages. Longan polysaccharide was relatively stable in the simulated intestinal juice system, but after digestion in simulated saliva and gastric juice, it degraded to a certain extent, releasing a small amount of reducing sugar dominated by glucose, while the digestion performance of IDLP was different from the other four polysaccharides. Dry preparation affects the physicochemical and biochemical properties of polysaccharides in longan pulp.

Key words: longan, polysaccharide, physicochemical characteristic, activity, digestion

CLC Number:

TS201.4

Wang ShiQi Yang YI. Comparison on the Physicochemical and Biochemical Characteristics of Crude Polysaccharides from Longan Pulps Dried by Different Methods[J]. FOOD SCIENCE, 0, (): 0-0.

References

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