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• Nutrition and Hygiene •     Next Articles

Repair Effect of Lactoferrin on Triadimefon Induced Damage of Bone Marrow Mesenchymal Stem Cells

1, 1,Yan XUHaowei REN1, Ning Liu   

  • Received:2019-07-19 Revised:2020-05-27 Online:2020-07-15 Published:2020-07-18
  • Contact: Ning Liu E-mail:ningliuneau@outlook.com

Abstract: The present study was designed to investigate the repairing effect of Lactoferrin (Lf) on triadimefon (TDF) induced damage of bone marrow mesenchymal stem cells (BMSCs). The cell damage model was induced by TDF. The CCK-8 assay was used to evaluate the proliferation of BMSCs. After being treated with TDF and Lf, the cells were collected for determining the activities of alkaline phosphatase (ALP) and osteocalcin (OCN), and the changes in osteogenesis and adipogenic gene expression. Detection of osteogenic differentiation ability using alizarin red staining and detection of adipogenic differentiation ability using oil red O staining. We found that compared with the blank control group, Lf at all concentrations tested promoted cell proliferation (P<0.05). Lf increased the cell survival rate, elevated the content of ALP and OCN in the cells, increased the formation of mineralized nodules, reduced the formation of lipid drople, as well as increased the relative expression of osteogenic differentiation-related genes, and decreased the relative expression of genes involved in adipogenic differentiation. According to our results, Lf can promote the proliferation of BMSCs damaged by TDF, differentiated the bone marrow mesenchymal stem cells into osteogenic direction, which can promote the formation of new bone better and consequently repair cell damage.

Key words: triadimefon, bone marrow mesenchymal stem cells (BMSCs), lactoferrin, differentiation, repair

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