Antibacterial Mechanism and Activity of Caryophyllene against Brochothrix thermosphacta

Abstract

Abstract: In this paper, the antibacterial effect and action mechanism of caryophyllene against Brochothrix thermosphacta was studied. The inhibitory activity was investigated by the determination of the minimum inhibitory concentration (MIC) and growth curve of caryophyllene against B. thermosphacta. The damage of cell morphology and cell wall structure by caryophyllene was determined by observing scanning electron microscopy (SEM) and the leakage of alkaline phosphatase (AKP). The effect on the cell membrane was examined by the leakage of potassium ions, fluorescein diacetate (FDA) and proteins. The effect on cell metabolism was investigated by measuring the activity of malate dehydrogenase (MDH) and pyruvate kinase (PK). The binding of caryophyllene to bacterial DNA was studied by fluorescence spectroscopy. The results showed that the MIC of caryophyllene against B. thermosphacta was 5 mL/L. 1×MIC and 2×MIC caryophyllene could destroy the morphology and structure of cells and the permeability of cell membrane, affecting cell metabolism, leading to a significant decrease in MDH and PK activities (P < 0.05), and destroying the conformation and structure of genomic DNA.

Key words: caryophyllene , brochothrix thermosphacta, antibacterial mechanism, malicdehydrogenase(MDH), pyruvate kinase (PK), genomic DNA

CLC Number:

TS201.3

Hui-Zhen SHU CHEN Wen-Xue. Antibacterial Mechanism and Activity of Caryophyllene against Brochothrix thermosphacta[J]. FOOD SCIENCE, 0, (): 0-0.

References

参考文献
[1] 曾莹. 天然防腐剂的筛选及其抗菌性研究[J]. 中国调味品, 1992(9):10-15.
[2] 张玉芬, 张森, 刘准. 食品保藏剂N—1081的抗菌作用[J]. 食品与发酵工业, 1986(5):64-66.
[3] 孔凡真. 冷却肉的腐败变质与保鲜包装技术[J]. 肉类工业, 2006(11):3-4.
[4] Jiang Y, Gao F, Xing-Lian X U, et al. Microfloral Change of Vacuum-packaged Pork during Chilled Storage[J]. Food Science, 2011,32(4):241-245.
[5] Reid R, Fanning S, Whyte P, et al. The microbiology of beef carcasses and primals during chilling and commercial storage[J]. Food Microbiology, 2017,61:50-57.
[6] Silbande A, Adenet S, Smith-Ravin J, et al. Quality assessment of ice-stored tropical yellowfin tuna (Thunnus albacares) and influence of vacuum and modified atmosphere packaging[J]. Food Microbiology, 2016,60:62-72.
[7] You-Wei A I, Wang H X, Xiong D P. Mechanism research of Brochothrix thermosphacta in protein degradation of chilled pork[J]. Food Science & Technology, 2015.
[8] 杜芸. 天然食品防腐剂的研究进展[J]. 食品研究与开发, 2006,27(3):67-71.
[9] 刘晓宇, 陈旭冰, 陈光勇. β-石竹烯及其衍生物的生物活性与合成研究进展[J]. 林产化学与工业, 2012,32(1):104-110.
[10] 陈晓龙, 陈光静, 柳中, 等. 海南黑、白胡椒有效成分的检测及其分析[J]. 中国调味品, 2017,42(11):102-106.
[11] 凌玮玮.牡荆化学成分及其抑菌活性研究[D]. 安徽农业大学, 2010.
[12] 曹奕.竹荪中抑菌物质的提取方法优化及其抑菌机理的初步探究[D]. 上海海洋大学, 2013.
[13] 贺松, 龚芳红, 张德纯, 等. 乳酸链球菌素对乳酸菌抑菌作用的研究[J]. 食品科学, 2009,30(23):352-355.
[14] Babii C, Bahrin L G, Neagu A N, et al. Antibacterial activity and proposed action mechanism of a?new class of synthetic tricyclic flavonoids[J]. Journal of Applied Microbiology, 2016,120(3):630-637.
[15] Diao W, Hu Q, Zhang H, et al. Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.)[J]. Food Control, 2014,35(1):109-116.
[16] 曾哲灵, 赵存洋, 罗春燕, 等. 单癸月桂酸甘油酯的抑菌作用及机理研究[J]. 食品科学, 2013,34(3):70-74.
[17] Miao J, Guo L, Chang K, et al. Inhibitory effects of a novel antimicrobial peptide from kefir against Escherichia coli[J]. Food Control, 2016,65:63-72.
[18] He M, Wu T, Pan S, et al. Antimicrobial mechanism of flavonoids against Escherichia coli ATCC 25922 by model membrane study[J]. Applied Surface Science, 2014,305:515-521.
[19] Amagliani G, Giammarini C, Omiccioli E, et al. Detection of Listeria monocytogenes using a commercial PCR kit and different DNA extraction methods[J]. Food Control, 2007,18(9):1137-1142.
[20] 李婷婷, 张慧芳, 姜杨, 等. 鲶鱼体表粘液粗提物对铜绿假单胞菌的抑菌机理初探[J]. 现代食品科技, 2015(7):67-73.
[21] Sánchez E, García S, Heredia N. Extracts of Edible and Medicinal Plants Damage Membranes of Vibrio cholerae[J]. Applied & Environmental Microbiology, 2010,76(20):6888-6894.
[22] Yao X, Zhu X, Pan S, et al. Antimicrobial activity of nobiletin and tangeretin against Pseudomonas[J]. Food Chemistry, 2012,132(4):1883-1890.
[23] Yamakawa, M. Moricin, a Novel Type of Antibacterial Peptide Isolated from the Silkworm, Bombyx mori[J]. Journal of Biological Chemistry, 1995,270(50):29923-29927.
[24] 卢群, 丘泰球, 杨红. Rh123、FDA荧光法测定超声作用对大肠杆菌细胞膜的影响[J]. 广东药学院学报, 2006,22(2):179-180.
[25] 黄巍, 王建清, 张倩. 香旱芹精油对金黄色葡萄球菌抑菌机理的研究[J]. 中国调味品, 2017,42(12):7-11.
[26] 朱力, 王恒樑, 黄培堂. 蛋白质组学在细菌应激反应研究中的应用[J]. 生物技术通讯, 2007,18(3):511-514.
[27] Takahashi-í?iguez Tóshiko, Aburto-Rodríguez Nelly, Vilchis-González Ana Laura, 等. 微生物苹果酸脱氢酶的功能、动力学特征、晶体结构以及调控[J]. Journal of Zhejiang University-Science B(Biomedicine & Biotechnology), 2016,17(4):247-261.
[28] 陈玉环, 万春鹏, 彭旋, 等. 桂枝主要抑菌活性成分对柑橘青霉病菌的作用机制研究[J]. 现代食品科技, 2016(10):45-51.
[29] Hu Y, Zhang J, Kong W, et al. Mechanisms of antifungal and anti-aflatoxigenic properties of essential oil derived from turmeric ( Curcuma longa L.) on Aspergillus flavus[J]. Food Chemistry, 2017,220:1-8.
[30] Yao X, Zhu X, Pan S, et al. Antimicrobial activity of nobiletin and tangeretin against Pseudomonas[J]. Food Chemistry, 2012,132(4):1883-1890.
[31] 叶陈毅, 陈炜平, 赵翔, 等. 丙酮酸激酶M2在肿瘤及骨关节炎发病中的作用[J]. 浙江医学, 2017,39(39):2057.
[32] 王志坤.酿酒酵母BY5419生产丙酮酸的定向进化及辅酶工程改造[D]. 山东大学, 2010.
[33] Pilane M C, Bagla V P, Mokgotho M P, et al. Free Radical Scavenging Activity: Antiproliferative and Proteomics Analyses of the Differential Expression of Apoptotic Proteins in MCF-7 Cells Treated with Acetone Leaf Extract of Diospyros lycioides (Ebenaceae)[J]. Evidence-Based Complementray and Alternative Medicine,2015,(2015-9-17), 2015,2015(12).
[34] Ning Y, Yan A, Yang K, et al. Antibacterial activity of phenyllactic acid against Listeria monocytogenes and Escherichia coli by dual mechanisms[J]. Food Chemistry, 2017,228:533-540.
[35] Tang Y L, Shi Y, Zhao W, et al. Interaction of MDpep9, a novel antimicrobial peptide from Chinese traditional edible larvae of housefly, with Escherichia coli genomic DNA[J]. Food Chemistry, 2009,115(3):867-872.
[36] Nyarko E, Hanada N, Habib A, et al. Fluorescence and phosphorescence spectra of Au(III), Pt(II) and Pd(II) porphyrins with DNA at room temperature[J]. Inorganica Chimica Acta, 2004,357(3):739-745.
[37] 陈奕鹏, 杨扬, 时涛, 等. 内生真菌HND5挥发性物质组分分析及其抑菌作用测定[J]. 热带作物学报, 2017,38(4):689-694.
[38] Ultee A, Kets E P, Smid E J. Mechanisms of Action of Carvacrol on the Food-Borne Pathogen Bacillus cereus[J]. Applied & Environmental Microbiology, 1999,65(10):4606-4610.