The Inhibition Effect of Lignosus Rhinocerotis Polysaccharides-Selenium nanoparticles on Non-enzymatic Glycosylation Under Ultrasound

Abstract

Abstract: Stable Lignosus rhinocerotis polysaccharides-selenium nanoparticles (LRP-SeNPs) were synthesized under ultrasound, and the effects of LRP-SeNPs on the characteristic products (fructosamine, dicarbonyl compounds and fluorescence end products) of non-enzymatic glycosylation reaction at three stages were studied by ultraviolet spectrum, fluorescence spectrum and SDS-PAGE. The results showed that ultrasound LRP-SeNPs (U-LRP-SeNPs) could effectively inhibit non-enzymatic glycosylation reaction with a certain dose dependent effect. U-LRP-SeNPs (Se/LRP=1/10 and 1/15) had good non-enzymatic glycosylation inhibition activity and the inhibition rate remained at 30% and 20%, respectively. The inhibition effect of U-LRP-SeNPs was further verified by SDS-PAGE. Ultrasound could effectively improve the stability of U-LRP-SeNPs, reduce the size of SeNPs, and increase the specific surface area. It provides more sites for binding with free radicals, so as to shield or terminate free radicals, effectively inhibits the oxidation reaction caused by free radicals, and prevents the non-enzymatic glycation reaction from the oxidation stage.

Key words: Lignosus rhinocerotis polysaccharides-selenium nanoparticles (LRP-SeNPs), ultrasound, non-enzymatic glycosylation, inhibition effect

CLC Number:

TS254.1

References

[1] 杨文英.中国糖尿病的流行特点及变化趋势[J].中国科学(生命科学), 2018, 48(8):812-819.
[2] Wang X, Zhang L S, Dong L L. Inhibitory effect of polysaccharides from pumpkin on advanced glycation end-products formation and aldose reductase activity[J]. Food Chemistry, 2012, 130(4):821-825. DOI:10.1016/j.foodchem.2011.07.064.
[3] Zhang L, Zhang C J, Tu Z C, et al. Nelumbo nucifera, leaf extracts inhibit the formation of advanced glycation end-products and mechanism revealed by Nano LC-Orbitrap-MS/MS[J]. Journal of Functional Foods, 2018, 42:254-261. DOI: 10.1016/j.jff.2018.01.012.
[4] Lee A Y W, Chung S S M. Contributions of polyol pathway to oxidative stress in diabetic cataract[J]. The FASEB Journal, 1999, 13(1):23-30. DOI: 10.0000/PMID9872926.
[5] Li Y, Li X, Wong Y S, et al. The reversal of cisplatin-induced nephrotoxicity by selenium nanoparticles functionalized with 11-mercapto-1-undecanol by inhibition of ROS-mediated apoptosis[J]. Biomaterials, 2011, 32(34):9068-9076. DOI: 10.1016/j.biomaterials.2011.08.001.
[6] Pan D, Liu J, Zeng X, et al. Immunomodulatory activity of selenium exopolysaccharide produced by Lactococcus lactis subsp. Lactis[J]. Food and Agricultural Immunology, 2015, 26(2):248-259. DOI: 10.1080/09540105.2014.894000.
[7] 胡婷.虎乳灵芝多糖的多级结构及溶液行为研究[D]. 武汉: 华中农业大学, 2017:38-59.
[8] Mallakpour S, Rashidimoghadam S. Application of ultrasonic irradiation as a benign method for production of glycerol plasticized-starch/ascorbic acid functionalized MWCNTs nanocomposites: Investigation of methylene blue adsorption and electrical properties[J]. Ultrasonics Sonochemistry, 2018, 40:419-432. DOI: 10.1016/j.ultsonch.2017.07.032.
[9] Yan J K, Pei J J, Ma H L, et al. Effects of ultrasound on molecular properties, structure, chain conformation and degradation kinetics of carboxylic curdlan[J]. Carbohydrate Polymers, 2015, 121:64-70. DOI:10.1016/j.carbpol.2014.11.066.
[10] Bi H, Gao T, Li Z, et al. Structural elucidation and antioxidant activity of a water-soluble polysaccharide from the fruit bodies of Bulgaria inquinans (Fries)[J]. Food Chemistry, 2013, 138(2-3):1470-1475. DOI: 10.1016/j.foodchem.2012.11.039.
[11] Liu M, Meng G, Zhang J, et al. Antioxidant and Hepatoprotective Activities of Mycelia Selenium Polysaccharide byHypsizigus marmoreusSK-02[J]. Biological Trace Element Research, 2016, 172(2):437-448. DOI: 10.1007/s12011-015-0613-z.
[12] Lee S, Tan N, Fung S, et al. Anti-inflammatory effect of the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden, the Tiger Milk mushroom[J]. BMC Complementary and Alternative Medicine, 2014, 14(1):359. DOI: 10.1186/1472-6882-14-359.
[13] Hu T, Huang Q, Wong K, et al. A hyperbranched β-d-glucan with compact coil conformation from Lignosus rhinocerotis sclerotia[J]. Food Chemistry, 2017, 225:267-275. DOI: 10.1016/j.foodchem.2017.01.034.
[14] Cai W, Hu T, Bakry A M, et al. Effect of ultrasound on size, morphology, stability and antioxidant activity of selenium nanoparticles dispersed by a hyperbranched polysaccharide from Lignosus rhinocerotis[J]. Ultrasonics Sonochemistry, 2018,42:823-831. DOI: 10.1016/j.ultsonch.2017.12.022.
[15] Zhang L S, Wang X, Dong L L. Antioxidation and antiglycation of polysaccharides from Misgurnus anguillicaudatus[J]. Food Chemistry, 2011, 124(1):183-187. DOI: 10.1016/j.foodchem.2010.06.006.
[16] Baker J R, Zyzak D V, Thorpe S R, et al. Chemistry of the fructosamine assay: D-glucosone is the product of oxidation of Amadori compounds[J]. Clinical Chemistry, 1994, 40(10):1950-1955. DOI: 10.1016/0009-9120(94)00046-8.
[17] Wells-Knecht K J, Zyzak D V, Litchfield J E, et al. Mechanism of autooxidative glycosylation: Identification of glyoxal and arabinose as intermediates in the autoxidative modification of proteins by glucose[J]. Biochemistry, 1995, 34(11):3702-3709. DOI:10.1021/bi00011a027.
[18] Wang Z B, Chen B B, Luo L, et al. Fractionation, physicochemical characteristics and biological activities of polysaccharides from Pueraria lobata roots[J]. Journal of the Taiwan Institute of Chemical Engineers, 2016(67):54-60. DOI:10.1016/j.jtice.2016.07.029.
[19] Ma J, Liang S, Wang Z, et al. ROCK pathway participates in the processes that 15-hydroxyeicosatetraenoic acid (15-HETE) mediated the pulmonary vascular remodeling induced by hypoxia in rat[J]. Journal of Cellular Physiology, 2010, 222(1):82-94. DOI: 10.1002/jcp.21923.
[20] 汤剑明, 洪莉. 氧化应激调控细胞外基质代谢的研究进展[J]. 中国医药导报, 2016, 13(4):36-40.
[21] 张良栓. 泥鳅多糖抗氧化及抗蛋白质非酶糖基化活性研究[D]. 哈尔滨: 哈尔滨医科大学, 2010:8-16.
[22] Wu J W, Hsieh C L, Wang H Y, et al. Inhibitory effects of guava (Psidium guajava L.) leaf extracts and its active compounds on the glycation process of protein[J]. Food Chemistry, 2009, 113(1):78-84. DOI: 10.1016/j.foodchem.2008.07.025.
[23] Wang X, Zhang L S, Dong L L. Inhibitory effect of polysaccharides from pumpkin on advanced glycation end-products formation and aldose reductase activity[J]. Food Chemistry, 2012, 130(4):821-825. DOI: 10.1016/j.foodchem.2011.07.064.
[24] Johnson R N, Metcalf P A, Baker J R. Fructosamine: A new approach to the estimation of serum glycosylprotein. An index of diabetic control[J]. Clinica Chimica Acta, 1983, 127(1):87-95. DOI: 10.1016/0009-8981(83)90078-5.
[25] Meade S J, Gerrard J A. The structure-activity relationships of dicarbonyl compounds and their role in the Maillard reaction[C]// International Congress Series, Kumamoto, Japan, October 29-November 01, 2001:455-456.
[26] Ruggiero-Lopez D, Lecomte M, Moinet G, et al. Reaction of metformin with dicarbonyl compounds. Possible implication in the inhibition of advanced glycation end product formation[J]. Biochemical Pharmacology, 1999, 58(11):1765-1773. DOI: 10.1016/S0006-2952(99)00263-4.
[27] Navarro-Alarcon M, Cabrera-Vique C. Selenium in food and the human body: A review[J]. Science of the Total Environment, 2008, 400(1-3):115-141. DOI:10.1016/j.scitotenv.2008.06.024.
[28] Ye S, Zhang J, Liu Z, et al. Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities[J]. Carbohydrate Polymers, 2016, 142:230-239. DOI: 10.1016/j.carbpol.2016.01.058.
[29] Zha X Q, Li X L, Zhang H L, et al. Pectinase hydrolysis of Dendrobium huoshanense polysaccharide and its effect on protein nonenzymatic glycation[J]. International Journal of Biological Macromolecules, 2013, 61:439-447. DOI: 10.1016/j.ijbiomac.2013.08.008.
[30] Fu M X, Wells-Knecht K J, Blackledge J A, et al. Glycation, Glycoxidation, and Cross-Linking of Collagen by Glucose: Kinetics, Mechanisms, and Inhibition of Late Stages of the Maillard Reaction[J]. Diabetes, 1994, 43(5):676-683. DOI:10.2337/diabetes.43.5.676.