FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (22): 110-117.doi: 10.7506/spkx1002-6630-20190520-225

• Bioengineering • Previous Articles     Next Articles

Immobilization of Transglutaminase and Its Application in the Treatment of Rice Bran Wastewater

PEI Xingwu, WANG Hong, GAO Ziwen, YUAN Taizeng, GAO Ming, YAO Kai, PAN Mingzhe, YU Dianyu   

  1. (1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Hubei Tianxing Grain and Oil Co. Ltd., Suizhou 441300, China)
  • Online:2019-11-25 Published:2019-12-02

Abstract: In this study, cellulose acetate-polypropylene composite membrane was prepared, and microbial transglutaminase (MTG) was immobilized onto the membrane, yielding an MTG-immobilized membrane, whose enzymatic activity was determined to be 17.6 U/g. Then the enzyme-loaded membrane was fixed on a stainless steel mesh frame and suspended in a beaker in order to use it to treat protein in rice bran wastewater. The effects of material temperature, material pH, rotor speed and enzyme-loaded membrane area on protein polymerization rate were investigated by one-factor-at-a-time method. Furthermore, using response surface optimization, the optimum polymerization conditions were obtained as follows: material temperature 43 ℃, pH 6.6, rotor speed 124 r/min, ratio between enzyme-loaded membrane area and substrate amount 80:1 (cm2/g), and reaction time 1.8 h. Under these conditions, protein polymerization rate was 70%. The enzyme-immobilized membrane remained above 74.3% of the original activity after its fifth repeated use. This study provides a theoretical basis for the continuous polymerization of proteins in rice bran wastewater and the retention of nutrients.

Key words: transglutaminase, enzyme-loaded composite membrane, rice bran wastewater, protein polymerization rate, repeated use

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