Comparative study on the stability of five lactic acid bacteria adsorbing acrylamide

Abstract

Abstract: Acrylamide (AA), a toxin that primarily exists in fried food, causes neurotoxicity, genetic toxicity, genetic mutations, and DNA damage.The study investigated the capacity of active bacteria and heat-inactivated bacteria of five strains lactic acid bacteria (LAB) in adsorbing acrylamide. At the same time, the stability of the lactic acid bacteria-acrylamide (LAB-AA) was evaluated in sterile deionized water, different organic solvents (methanol, acetonitrile/water and ketone), and in-vitro simulated gastrointestinal environment, respectively. The AA adsorption rate of five strains LAB in the heat-inactivated group was found to be higher than that in the active group. The peak AA adsorption rates of the heat-inactivated and active L. plantarum ATCC8014 were determined to be 96.53% .Five LAB-AA complexes were eluted three times with organic solvents, but no AA was released. After sterile deionized water eluted, a small amount of AA was released.Simulated gastrointestinal environmental tests confirmed that pH , bile salt , trypsin and time of action would affect the stability of LAB-AA complexes, resulting in a small amount of AA release.In conclusion,the heat-inactivated L. plantarum ATCC8014 not only has the best adsorption capacity, but also has better adsorption stability.These results provide a new idea for the toxicity inhibition of acrylamide in food.

Key words: Acrylamide, lactic acid bacteria, adsorption capacity, gastrointestinal environment, stability

CLC Number:

TS201.6

References

[1]Jin J, Wu X, Zhang Y.Relationship between antioxidants and acrylamide formation: a review[J].Food Research International, 2013, 51(2):611-620 [2]Mottram D S, Wedzicha B L, Dodson A T.Acrylamide is formed in the Maillard reaction[J].Nature, 2002, 419(6906):448-449 [3] Krishna G, Muralidhara.Inulin supplementation during gestation mitigates acrylamide-induced maternal and fetal brain oxidative dysfunctions and neurotoxicity in rats. [J].Neurotoxicology and Teratology, 2015, 49(May–June 2015):49-58 [4]Olsen A, Christensen J, Outzen M, et al.Pre-diagnostic acrylamide exposure and survival after breast cancer among postmenopausal Danish women[J].Toxicology, 2012, 296(1-3):67-72 [5]Chain E.Scientific opinion on acrylamide in food[J].EFSA Journal, 2015, 13(6):4104-4104 [6]Yang H J, Lee S H, Choi J H, et al.Toxicological effects of acrylamide on rat testicular gene expression profile[J].Reproductive Toxicology, 2005, 19(4):527-534 [7]Franco P, Karl K, Kit G.Reduction of acrylamide formation in potato slices during frying[J].Wissenschaft and Technologie, 2004, 37(6):679-685 [8]Rydbery P, Eriksson S, Tareke E, et al.Investigation of factors that influence the acrylamide content of heated foodstuffs[J].Journal of Agricultural and Food Chemistry, 2003, 51(24):7012-7018 [9]Bovo F, Franco L T, Rosim R E, et al.In vitro ability of beer fermentation residue andyeast-based products to bind aflatoxin B1[J].Brazilian Journal of Microbiology, 2015, 46(2):577-581 [10]Anese M, Bortomeazzi R, Manzocco L, et al.Effect of chemical and biological dipping on acrylamide for mationand sensory properties in deep-fried potatoes[J].Food Research International, 2009, 42(1):142-147 [11] Chen Y, Kong Q, Chi C, et al.Biotransformation of aflatoxin B1 and aflatoxin G1 in peanut meal by anaerobic solid fermentation of Streptococcus thermopHilus and Lactobacillus delbrueckii subsp. Bulgaricus. [J].International Journal of Food Microbiology, 2015, 211(15):1-5 [12]Kachouri F, Ksontini H, Hamdi M.Removal of aflatoxin B1 and inhibition of Aspergillus flavus growth by the use of Lactobacillus plantarum on olives[J].Journal of Food Protection, 2014, 77(10):1760-1767 [13]Corassin C H, Bovo F, Rosim R E, et al.Efficiency of Saccharomyces cerevisiae,and lactic acid bacteria strains to bind aflatoxin M1 in UHT skim milk[J].Food Control, 2013, 31(1):80-83 [14]Piotrowska M.The Adsorption of Ochratoxin A by Lactobacillus Species[J].Toxins, 2014, 6(9):2826-2839 [15]Ambalam P, Dave J M, Nair B M, et al.In vitro mutagen binding and antimutagenic activity of human Lactobacillus rhamnosus231[J].Anaerobe, 2011, 17(5):217-222 [16]Abbès S, Ben S J, Jebali R, et al.Interaction of aflatoxin B1 and fumonisin B1 in mice causes immunotoxicity and oxidative stress: Possible protective role using lactic acid bacteria[J].Journal of Immunotoxicology, 2015, 13(1):46-54 [17]Wang L, Yue T, Yuan Y, et al.A new insight into the adsorption mechanism of patulin by the heat-inactive lactic acid bacteria cells.[J].Food Control, 2015, 50(April 2015):104-110 [18] Zhai Q, Yin R, Yu L, et al.Screening of lactic acid bacteria with potential protective effects against cadmium toxicity.[J].Food Control, 2015, 54(August 2015):23-30 [19]张梦梅, 刘书亮.乳酸菌吸附重金属的影响因素、机理及应用研究进展[J].食品科学, 2018, 39(15):316-322 [20]Zoghi A, Khosravi-Darani K, Sohrabvandi S.Surface binding of toxins and heavy metals by probiotics[J].Mini- Reviews in Medicinal Chemistry, 2014, 14(1):84-98 [21] Sangsila A, Faucet-Marquis V, Pfohl-Leszkowicz A, et al.Detoxification of zearalenone by Lactobacillus pentosus strains. [J]. Food Control, 2016, 62(April 2016):187-192 [22]Lahtinenyz S J, Haskard C A, Ouwehandz A C, et al.Binding of aflatoxin B1 to cell wall components of Lactobacillus rhamnosus strain GG[J].Food Additives & Contaminants, 2004, 21(2):158-164 [23] Shen Y, Zhao S J, Zhao X D, et al.In vitro adsorption mechanism of acrylamide by lactic acid bacteria.[J].LWT - Food Science and Technology, 2019, 100(2019):119-125 [24]Zhang D, Liu W, Li L, et al.Key role of peptidoglycan on acrylamide binding by lactic acid bacteria[J].Food Science & Biotechnology, 2017, 26(1):271-277 [25]Elnezami H, Kankaanp?? P, Salminen S, et al.Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media[J].Journal of Food Protection, 1998, 61(4):466-468 [26]Haskard C A, Elnezami H S, Kankaanp?? P E, et al.Surface binding of aflatoxin B1 by lactic acid bacteria[J].Applied & Environmental Microbiology, 2001, 67(7):3086-3091 [27]Serrano-Ni?o J C, Cavazos-Gardu?o A, Cantú-Cornelio F, et al.In vitro reduced availability of aflatoxin B1 and acrylamide by bonding interactions with teichoic acids from lactobacillus strains[J].LWT - Food Science and Technology, 2015, 64(2):1334-1341 [28]Haskard C, Binnion C, Ahokas J.Factors affecting the sequestration of aflatoxin by Lactobacillus rhamnosus strain GG[J].Chem Biol Interact, 2000, 128(1):39-49 [29] Zhao H, Wang X, Zhang J, et al.The mechanism of Lactobacillus strains for their ability to remove fumonisins B1 and B2.[J].Food and Chemical Toxicology, 2016, 97(2016):40-46 [30]Begley M，Gahan C，Hill C.The interaction between bacteria and bile[J].FEMS Microbiology Review, 2005, 29(4):625-651