FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (1): 9-15.doi: 10.7506/spkx1002-6630-20190623-270

• Basic Research • Previous Articles     Next Articles

Separation, Purification and Biochemical Properties of Exopolysaccharides from Weissella confusa

JIANG Jing, DU Renpeng, GUO Shangxu, ZHAO Dan   

  1. (1. Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China; 2. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Harbin 150080, China)
  • Online:2020-01-15 Published:2020-01-19

Abstract: A strain of lactic acid bacteria (LAB) with high exopolysaccharides (EPS)-producing ability was isolated from naturally fermented melon. The strain was identified as Weissella according to its physiological and biochemical properties. In addition, the chemical composition, solubility, water-holding capacity, emulsifying property, antibacterial property and antioxidant activity of the purified EPS from this strain were determined. The results showed that the total sugar, protein and uronic acid contents were (93.67 ± 2.08)%, (0.32 ± 0.01)% and (6.01 ± 0.34)%, respectively. The dissolution percentage and water holding capacity of the EPS were (98.78 ± 1.37)% and (426.03 ± 7.26)%, respectively. It exhibited good emulsifying, bacteriostatic and in vitro antioxidant properties in a concentration-dependent manner.

Key words: Weissella confusa, exopolysaccharide, separation and purification, biochemical properties

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