FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (2): 114-118.doi: 10.7506/spkx1002-6630-20181229-356

• Bioengineering • Previous Articles     Next Articles

Effects of Different Fermentation Conditions on Folate Synthesis of Lactobacillus plantarum

LI Qiangkun, LIU Chenjian, LUO Yiyong, YANG En, LI Xiaoran   

  1. (Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China)
  • Online:2020-01-25 Published:2020-01-19

Abstract: In this study, Lactobacillus plantarum 4-3, producing folate at high yield, was investigated for the effects of natural pH and maintaining pH at 6.0 on its growth status and folate synthesis in skim milk or soybean milk. The results showed that strain 4-3 produced more folate when cultured in soybean milk. When the culture pH was controlled at 6.0, this strain could still maintain high cell density after the logarithmic growth phase and showed enhanced accumulation of folate. Real-time fluorescence quantitative polymerase chain reaction (qPCR) results showed that the expression levels of folate biosynthesis-related genes were higher at constant pH.

Key words: Lactobacillus plantarum, folate, fermentation substrate, pH

CLC Number: