Effect of HSP27 on myofibrillar proteins and caspases on Tibetan sheep meet tenderness during postmortem aging

Abstract

Abstract: In order to study the effects of heat shock protein 27 (Hsp27) on the degradation of myofibrillar proteins and the activity of apoptotic enzymes during the postmortem maturation of Tibetan mutton. The Tibetan mutton treated with Hsp27 inhibitor KRIBB3 was used as the research object to determine the activity changes of Caspase-3 and 9 and the solubility and degradation characteristics of myofibrillar protein during postmortem maturation of Tibetan mutton, and further study by in vitro test. Effect of Hsp27 on the degradation of myofibrillar protein by μ-calcium activase and Caspase-3. The results showed that Hsp27 significantly inhibited the activity of Caspase-3 and 9 (p < 0.05), inhibited the formation of myofibril fragmentation and the degradation of myologin and troponin-T. In vitro studies found that Hsp27 was able to Significant inhibition of μ-calcium activase and Caspase-3 degradation of myofibrillar proteins.

Key words: Tibetan sheep meat, Heat shock protein 27 (Hsp27), Caspases, Western blotting, Myofibrillar

CLC Number:

References

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