FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (4): 293-299.doi: 10.7506/spkx1002-6630-20180927-295

• Safety Detection • Previous Articles     Next Articles

Development of a Surface Plasmon Resonance Immunosensor for Detecting Olaquindox

XIE Chunhua, LIU Shuang, WANG Minsi, WANG Xialin, WANG Shen, ZHANG Zhijun, SONG Yang   

  1. (1. Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China; 2. National Engineering and Technology Research Center for Preservation of Agricultural Products (Tianjin), Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Tianjin 300384, China)
  • Online:2020-02-25 Published:2020-03-02

Abstract: A sensitive and stable competitive surface plasmon resonance (SPR) immunosensor was established and used to detect olaquindox (OLA) in feed and aquaculture water samples. Its sensitivity and limit of detection (LOD) were 4.7 and 0.5 μg/L, respectively. The best regeneration efficiency was obtained with 2.5 mmol/L NaOH solution, the chip could be reused 60 times and the detection time was only 10 min. The results of cross-reaction between OLA and structural analogs showed a high sensitivity and specificity. Additionally, good recoveries (84.7%–99.3%) and coefficients of variation (1.2%–10.1%) were obtained for spiked fish, shrimp feed and aquaculture water samples. When the method was applied to test actual samples of feed and culture water, two positive samples were detected. OLA contents in fish culture water and chicken feed were 5.0 and 6.1 μg/L, respectively. The method can efficiently detect OLA in large-volume feed and water samples, and has the advantages of short detection time, low detection limit, high accuracy and sensitivity, and convenient operation.

Key words: olaquindox, surface plasmon resonance, immunosensor, feed, water

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