Abstract: In order to rapidly detect residual amantadine and chloramphenicol residues in poultry meats, a indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) was mainly developed for the simultaneous detection of amantadine and chloramphenicol by the efficient sample pretreatment. After optimizing the dilution multiple of coating antigen and antibody, competitive time and the dilution multiple of second antibody, the half-maximum inhibition concentration (IC50) of amantadine was 0.33 μg/L and the detection range was 0.06 μg/L~1. 77 μg/L. The IC50 of CAP is 0.04 μg/L and the detection range is 0.010 μg/L~0.179 μg/L. Sample was extracted by ethyl acetate and potassium carbonate solution, and then the extraction liquid was blown dry with nitrogen. After purified by n-hexane, the extract was determined. The recoveries of amantadine and chloramphenicol were 92.55%~105.14% and 92.00%~112.50%, respectively, and the coefficients of variation were both less than 10.48%. Furthermore, the result determined by ic-CLEIA were very compatible with that of instrument analysis method (R2>0.98),which demonstrated that the established ic-CLEIA methods have high accuracy and good reliability. This method would provide a reference for the rapid detection of drug residues and the corresponding pretreatment techniques.

Key words: poultry meat, amantadine, chloramphenicol, ic-CLEIA, pretreatment