Screening of the protease-producing strain and optimization of enzyme-producing conditions using rapeseed meal as a nitrogen source

Abstract

Abstract: In this study, a high-protease strain named CL-10 was screened from the canteen sewage in Nanchang University, and was identified as Bacillus amyloliquefaciens through physiological and biochemical experiments and 16S rRNA sequencing technology. Using the protease activity of Bacillus amyloliquefaciens as the response value, a single factor experiment was applied to optimize the on the components of enzyme-producing culture medium whose nitrogen source was rapeseed meal. Besides, the contents of rapeseed meal, corn meal and bran were particularly optimized by response surface method. As a result, the optimal components were as follow: the rapeseed meal content of 6.1%, the corn flour content of 4.4%, the bran content of 3.2%, the Tween 20 concentration of 0.7%, and the ZnSO4 · 7H2O concentration of 0.2%. On this condition, the maximum activity was up to 6385.1U / ml, which was 1.35 times higher than that of 2715U / ml in the basic fermentation medium.

Key words: strain screening, protease, Bacillus amyloliquefaciens, rapeseed meal, response surface optimization

CLC Number:

Q815

References

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