Abstract: Objective: An effective method of ultra performance liquid chromatography-mass spectrometry/mass spectrometry ( UPLC- MS/MS) was established for rapid screening of 27 plant growth regulator and antibiotic residues in bean sprouts. Methods: The chemical drugs were separated on an Agilent Eclipse Plus C18 chromatographic column ( 2.1 mm × 100 mm，1.8 μm) with methanol and 0.01% formic acid as mobile phase under gradient elution at a flow rate of 0.4 mL/min.The 27 plant growth regulator and antibiotic residues in bean sprouts were conducted qualitatively and quantitatively analysis by tandem mass spectrometry under multiple reaction monitoring(MRM) mode. Results: 27 linear calibration curves were obtained with correlation coefficients which were more than 0.9989, and the precision expressed as relative standard deviations ranged from 1.1% to 3.5%(n＝6). The recoveries of plant growth regulator and antibiotic residues in bean sprouts at different spiked concentrations ranged from 80.5% to 114.9%. The qualitative and quantitative limits were at 0.5 – 50 μg/kg and 1.5 - 150μg/kg. Conclusion: This method is rapid, accurate and sensitive with a high degree of separation method which can be used for the detection of the 27 plant growth regulator and antibiotic residues in bean sprouts.

Key words: UPLC- MS/MS, Bean Sprouts, Plant Growth Regulator, Antibiotic Residues