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• Nutrition and Hygiene •     Next Articles

Anti-aging effects of SIRT3-augmenting polyphenols on UVB-irradiated HaCaT cells

  

  • Received:2019-12-30 Revised:2020-12-15 Online:2021-03-15 Published:2021-03-22
  • Contact: chong zhao E-mail:zhaoch0206@cau.edu.cn
  • Supported by:
    Youth Program of National Natural Science Foundation of China

Abstract: Abstract: SIRT3 is a deacetylase located in the mitochondrial matrix, which plays an important role in mitochondrial energy metabolism, oxidative stress regulation and anti-aging. In this experiment, SIRT3- EGFP gene report system and qRT-PCR were performed to screen SIRT3 activators from eight polyphenols including Resveratrol, Kaempferol, Punicalin, Punicalagin, Fisetin, Cafestol, Cyanidin 3-glucoside chloride, Delphinidin 3-glucoside chloraid. Futhermore, the anti-aging effects of SIRT3-augmenting polyphenols were evaluated by UVB-irradiated HaCaT cells model. Our results showed that Resveratrol, Kaempferol, Punicalagin, Fisetin and Cafestol significantly enhanced the gene expression of SIRT3. Resveratrol, Punicalagin, Fisetin and Cafestol effectively reduced UVB-induced intracellular ROS levels. Furthermore, these five polyphenols all significantly increased the GSH/GSSG ratio, but only Resveratrol and Cafestol significantly enhanced the SOD2 activity. In addition, Punicalagin not only activated SIRT3 and repaired the cell antioxidant system, but also significantly decreased the activity of SA-β-gal, a marker of cell senescence. In conclusion, Resveratrol, Kaempferol, Punicalagin, Fisetin and Cafestol all could augmented SIRT3 gene expression. Among them, Punicalagin significantly repaired UVB-induced cell senescence in HaCaT cells, and its anti-aging mechanism may be related to the antioxidant pathway of SIRT3-NADPH-GSH/GSSG.

Key words: Keywords: SIRT3, polyphenol, anti-aging, oxidative stress

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