Study on Antibacterial Mechanism of Antimicrobial Peptide Brevilaterin Combined with ε-Polylysine for Staphylococcus aureus

Abstract

Abstract: Staphylococcus aureus is one common foodborne pathogenic bacterium, and the control of S. aureus is of great importance for the improvement of food safety. Thus, using S. aureus as indicator microorganism, the synergistical antibacterial mechanism of brevilaterin and ε-polylysine was investigated. The combined antibacterial mechanism was studied from the aspects of membrane proton motive force, cell membrane integrity, cell metabolism, cell ultrastructure, protein and nucleic acid synthesis. The results indicated that brevilaterin could dissipate the membrane potential, damage cell membrane integrity, result in the leakage of cell content, then enter into cells to inhibit the activity of respiratory chain dehydrogenase to inhibit S. aureus. ε-Polylysine could increase the membrane permeability, change the transmembrane proton gradient, enter into cells to degrade the protein or inhibit the synthesis of protein, and bind with DNA to inhibit the function of DNA, thereby to play the antibacterial activity. The combination of brevilaterin and ε-polylysine could synergistically damage the membrane integrity, inhibit the activity of respiratory chain dehydrogenase caused by brevilaterin, and bind with DNA due to ε-polylysine, finally to achieve multi-target synergistic antibacterial action.

Key words: Brevilaterin, ε-Polylysine, synergistic antibacterial mechanism, Staphylococcus aureus

CLC Number:

TS201.3

Zhi-Xin WANG. Study on Antibacterial Mechanism of Antimicrobial Peptide Brevilaterin Combined with ε-Polylysine for Staphylococcus aureus[J]. FOOD SCIENCE, 0, (): 0-0.

References

[1] OMWENGA I, ABOGE G O, MITEMA E S, et al.Staphylococcus aureus enterotoxin genes detected in milk from various livestock species in northern pastoral region of Kenya[J].Food Control, 2019, 103(0):126-132 [2]WATTINGER L, STEPHAN R, LAYER F, et al.Comparison of Staphylococcus aureus isolates associated with food intoxication with isolates from human nasal carriers and human infections[J].European Journal of Clinical Microbiology & Infectious Diseases Official Publication of the European Society of Clinical Microbiology, 2012, 31(4):455-64 [3]马俊美, 宁亚维, 王志新, 等.侧孢短芽孢杆菌抗菌肽的结构与性质[J].食品与生物技术学报, 2016, 35(6):629-634 [4] 宁亚维, 李文蕾, 苏丹, 等.抗菌肽brevilaterin与食品防腐剂联用对食源性致病菌的抑制[J].食品与发酵工业, 2019, 0(0):1-9 [5] CHHEDA A H, VERNEKAR M.A natural preservative ε-poly-L-lysine: Fermentative production and applications in food industry[J].International Food Research Journal, 2015, 22(0):23-30 [6] LIN Lin, GU Yulei, LI Changzhu, et al.Antibacterial mechanism of ε -Poly-lysine against Listeria monocytogenes and its application on cheese[J]. [J].Food Control, 2018, 91(0):76-84 [7]周祺，刘芳，范晓然，都立辉.聚赖氨酸对肠球菌的抑菌作用及机制[J].中国食品学报, 2018, 18(01):65-72 [8]刘蔚，周涛.聚赖氨酸抑菌机理研究[J].食品科学, 2009, 30(9):15-20 [9] LI Yingqiu, HAN Qing, FENG Jianling, et al. .Antibacterial characteristics and mechanisms of e-poly-lysine against Escherichia coli and Staphylococcus aureus[J]. [J].Food Control, 2014, 43(0):22-27 [10] 贾士儒.生物防腐剂[M]. 中国轻工业出版社, 2009: 132. [11]YE Ruosong, XU Hengyi, WAN Cuixiang, et al.Antibacterial activity and mechanism of action of epsilon-poly-L-lysine[J].Biochemical and Biophysical Research Communications, 2013, 439(1):148-153 [12]HENZLER WILDMAN K A, LEE D K, RAMAMOORTHY A.Mechanism of Lipid Bilayer Disruption by the Human Antimicrobial Peptide,LL-37[J].Biochemistry, 2003, 42(21):6545-6558 [13]POWERS J P S, MARTIN M M, GOOSNEY D L, et al.The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment[J].Antimicrobial Agents & Chemotherapy, 2006, 50(4):1522-1522 [14]CASTILLO F J D, CASTILLO I D, MORENO F.Construction and characterization of mutations at codon 751 of the Escherichia coli gyrB gene that confer resistance to the antimicrobial peptide microcin B17 and alter the activity of DNA gyrase[J].Journal of Bacteriology, 2001, 183(6):2137-2137 [15]PATRZYKAT A, FRIEDRICH C L, ZHANG L, et al.Sublethal concentrations of pleurocidin-derived antimicrobial peptides inhibit macromolecular synthesis in Escherichia coli[J].Antimicrobial Agents Chemotherapy, 2002, 46(3):605-614 [16] 郭娟，贠建民，邓展瑞，等.Bacillus pumilus HN-10抗真菌肽P-1对Trichothecium roseum的抑菌机理[J].食品科学, 2019, 0(0):1-9 [17] 贾英民, 李兴峰, 王志新, 等.一种从发酵液中快速提取食用抗菌肽的方法[P]. ZL 201210019936.X. [18] 冯建岭.ε-聚赖氨酸的防腐效果及对大肠杆菌抑制机理的研究[D]. 齐鲁工业大学, 2014. [19] RIAZI S, DOVER S E, CHIKINDAS M L.Mode of action and safety of lactosporin, a novel antimicrobial protein produced by Bacillus coagulans ATCC 7050[J]. [J].Journal of Applied Microbiology,, 2012, 113(3):714-722 [20]FARHA M A, VERSCHOOR C P, BOWDISH D, et al.Collapsing the proton motive force to identify synergistic combinations against Staphylococcus aureus[J].Chemistry & Biology, 2013, 20(9):1168-1178 [21] LEE J, KIM S, SIM J Y, et al.A potent antibacterial activity of new short d-enantiomeric lipopeptide against multi drug resistant bacteria[J]. [J].Biochimica et Biophysica Acta(BBA)- Biomembranes, 2018, 1(1):1-9 [22] WANG Yao, QIN Yuxuan, ZHANG Ying, et al.Antibacterial mechanism of plantaricin LPL-1, a novel class IIa bacteriocin against Listeria monocytogenes[J].[J]. Food Control, 2019, 97(0):87-93 [23]RONCEVIC T, KRCE L, GERDOL M, et al.Membrane-active antimicrobial peptide identified in Rana arvalis by targeted DNA sequencing[J].Biochimica et Biophysica Acta(BBA)- Biomembranes, 2019, 1861(3):651-659 [24]侯温甫, 欧阳何一, 吴忌, 等.曲酸对冷鲜鸭肉中优势腐败菌的抑制作用及其抑菌机理[J].食品科学, 2019, 40(01):286-293 [25]HYLDGAARD M, MYGIND T, VAD B S, et al.The antimicrobial mechanism of action of epsilon-poly-l-lysine[J].Applied and Environmental Microbiology, 2014, 80(24):7758-7770 [26] 韩晴.ε-聚赖氨酸对金黄色葡萄球菌的抑制机理及在牛乳上的应用效果[D]. .齐鲁工业大学, 2015, :- [27] LIU Hongxia, PEI Houbao, HAN Zhinan, et al.The antimicrobial effects and synergistic antibacterial mechanism of the combination of ε-Polylysine and nisin against Bacillus subtilis[J]. [J].Food Control, 2015, 47(0):444-450