FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (6): 252-258.doi: 10.7506/spkx1002-6630-20190527-334

• Processing Technology • Previous Articles     Next Articles

Decolorization and Antioxidant Activities of Peptides from Neptunea cumingi Muscle Protein Hydrolysate

ZHANG Shanshan, LI Xiaobin, ZHANG Xuanming, HAN Liwen, LIU Kechun   

  1. (Shandong Provincial Engineering Laboratory for Biological Testing Technology, Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China)
  • Online:2020-03-25 Published:2020-03-23

Abstract: Crude peptides were prepared from Neptunea cumingi muscle by using acid-alcohol extraction. By using the response surface methodology, the conditions for decolorizing the crude peptides were optimized as follows: activated carbon amount 0.42%, reaction temperature 52 ℃, reaction time 50 min and pH 7.4. Under these conditions, the retention rate of peptide was (67.63 ± 0.80)% and the decolorization rate was (68.70 ± 0.54)%. Furthermore, antioxidant activities of the purified peptides were evaluated both in vitro and in vivo. The half maximal effective concentration (EC50) for 1,1-diphenyl-2-trinitrobenzene hydrazine (DPPH) radical scavenging was 170.041 μg/mL. The in vivo antioxidant tests showed that the peptides showed similar activity to the positive control VC at a concentration of 50 μg/mL in a zebrafish model. In addition, at 200 μg/mL, the peptides could inhibit oxidative damage induced by metronidazole. The total amino acid content of the peptides was 853.46 mg/g. The amino acid composition was determined by precolumn derivatization. It was found that alkaline and acidic amino acids together accounted for about 80% of the total amino acids, and arginine, alanine and proline were the most abundant amino acids.

Key words: Neptunea cumingi-derived peptide, decolorization, response surface methodology, amino acid composition, antioxidant activities, zebrafish

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