Preparation of oxidized starch microgels encapsulating β-carotene by limited-region crystallization

Abstract

Abstract: This is the first report for using limited-region crystallization to prepare the β-carotene loaded oxidized starch microgels. Firstly, the β-carotene was dissolved in a volatile organic solvent as the inner oil phase (O1), then O1 was added into the aqueous oxidized starch solution (W) to form pre-emulsion. The emulsion was mixed with the FeSO4·7H2O solution and dropwise added into the outer oil phase (O2) to form O1/W/O2 double emulsion with shear mixing. Fe2+ was oxidized to Fe3+ by oxygen, and used to cross-link with COO- on oxidized starch to form microgels of 15.2±2.1 μm. The internal oil phase (O1) was volatilized and β-carotene was crystallized in the oxidized starch microgels. In this paper, the optimal conditions of organic solvents, concentrations of oxidized starch, and amounts of cross-linking agent were explored. Considering the solubility of β-carotene in the organic solvent, dichloromethane was selected as the internal oil phase (O1). The results showed that, the starch microgels had high loading efficiency up to 29±3.2% when the concentration of the oxidized starch solution was 10% and the molar ratio of COO- to Fe3+ was 4.58. β-carotene loaded in the microgels were stable in pH 3 and easily released in an intestinal alkaline environment. The oxidized starch microgels can be a potential low-cost food delivery system to improve the stability, solubility and bioavailability of fat-soluble functional ingredients.

Key words: β-carotene, oxidized starch, microgel, carrier, bioavailability

CLC Number:

TS201.2

References

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