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Transcriptome Analysis of Hansenula polymorpha DL-1 with Sodium Selenite Induced Biosynthesis and Accumulation of Glutathione

  

  • Received:2020-01-22 Revised:2021-01-04 Online:2021-03-25 Published:2021-03-22

Abstract: Abstract:To illustrate the mechanism of glutathione biosynthesis by Hansenula polymorpha DL-1 exposed to sodium selenite (Na2SeO3), differentially expressed genes (DEGs) in the Na2SeO3-treated and untreated conditions were explored using a combination of transcriptomic sequencing and bioinformatic methods.The results showed that 60 μmol/L Na2SeO3 was found to favor the increase in GSH yield by the yeast cells, and the levels of GSH reached 530.22 ± 9.6 mg/L. A total of 1254 distinct differential expression genes (DEGs) was identified in the Na2SeO3 induced group, of which 630 genes were up-regulated, whereas the remaining 624 genes were down-regulated. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of DEGs indicated that the genes involved in cell cycle, ribosome components and synthesis of biological molecules (amino acid, glycolysis, fat and nucleic acid) were up-regulated. In addition, genes encoding the synthase and kinase involved in the metabolism of glutathione and energy substances were up-regulated. This study provide adequate information for a better understanding of the physiological mechanism of GSH biosynthesis by H polymorpha, which also provided a theoretical support for subsequent molecular improvement of over-production GSH engineering strains.

Key words: Hansenula polymorpha, glutathione, sodium selenite, transcriptome, differential expression gene

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