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• Nutrition and Hygiene •     Next Articles

Antioxidant Effect of Passion Fruit Peel and Its Effect on Inhibiting Glucose Absorption in Vitro and Regulating Intestinal Microflora Structure in Hyperglycemic Rats

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  • Received:2020-02-26 Revised:2021-01-15 Online:2021-03-15 Published:2021-03-22

Abstract: In this paper,the passion fruit peel (Passion Fruit Peel, PFP), passion fruit peel extractive(Passion Fruit Peel Extractive, PFPE), passion fruit peel residues(Passion Fruit Peel Residues, PFPR), passion fruit peel soluble`` fiber extractive (Passion Fruit Soluble Dietary Fiber, PFSF) and passion fruit peel insoluble fiber extractive fiber(Passion Fruit Insoluble Dietary Fiber, PFIF) as raw material, the effects of thyme peel on the inhibition of digestive enzyme activity, delayed absorption of glucose, antioxidation and the regulation of intestinal flora in hyperglycemic rats were evaluated in vitro. The results showed that PFPE had strong antioxidant capacity against DPPH· and ABTS·+, and had strong inhibitory effect on α - glucosidase and α - amylase, with IC50 of 1.89 mg/ml, 32.25 mg/ml, 6.23 mg/ml and 28.65 mg/ml, respectively; combined with component analysis,it was found that the total phenolic and flavonoid components in PFPE were the main parts of its antioxidation and enzyme activity inhibition; at the same time, thyme peel also had delayed absorption of glucose, and dietary fiber, especially PFSF, made a major contribution. In the experiment of fermentation in vitro,thyme peel powder and its extracts were fermented by the fecal intestinal flora of hyperglycemic rats to produce short chain fatty acids,of which PFSF had the most significant effect; the statistical results of intestinal flora showed that the extract and pericarp powder of passion fruit could enrich Lactobacillus and Bifidobacterium,inhibit Escherichia coli and sulfate reducing bacteria;among them,PFSF has the best effect on promoting the growth of probiotics,and PFPE has better effect on inhibiting pathogens. In this study, the functional activity and main functional parts of passion fruit peel powder were determined. The purification and identification of polyphenol activity could improve the functional activity of passion fruit peel, which laid a foundation for studying the hypoglycemic effect and mechanism of passion fruit peel from the perspective of intestinal flora, and provided a theoretical basis for the functional food development of passion fruit peel.

Key words: passion fruit peel, antioxidant, intestinal flora, glucose absorption, polyphenol, dietary fiber

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