Abstract: Objective: In order to provide a theoretical basis for the scientific utilization of mulberry leaf, it is explored whether mulberry leaf alkaloids can improve the abnormal glucose and lipid metabolism and liver injury induced by oxidative stress. Method: Two hundred and twenty male Kunming mice were randomly divided into six groups, model group, positive control group (200 mg/kg of glutathione) and mulberry alkaloids low-dose, medium -dose and high-dose (50,100, 200 mg/kg?BW). To establish a oxidative damage model by using intraperitoneal 1000 mg/kg?BW D-galactose for 20d. The changes of fasting blood glucose, lipid level and liver injury were measured at weekends of 0, 4, 8 and 12. Results: In the experimental period and dose range of this study, com- pared with model group, the fasting blood glucose, the total cholesterol (TC), triglycerides (TG), the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) were significantly decrease in the positive control group and three dose of mulberry alkaloids groups(P＜0.05). Meanwhile, there was no significant difference between the high dose group of mulberry alkaloids and the positive drug group, and both recovered to the normal level (P＞0.05). The results of histopathological showed that the degree of liver lesions of positive control group and three mulberry alkaloids doses were significantly reduced. Moreover, the effect of mulberry leaf alkaloids and positive drugs on the abnormal glucose and lipid metabolism and liver injury in mice was dose-dependent but had no time effect. Conclusion: Mulberry alkaloids can improve glucose and lipid metabolism abnormalities and liver damage caused by oxidative stress, and its mechanism may be related to inhibiting the occurrence and developmen -t of oxidative stress.

Key words: mulberry alkaloid, oxidative stress, glucolipid metabolism, liver injury, improvement effect