FOOD SCIENCE ›› 0, Vol. ›› Issue (): 0-0.

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Determination of Cinnamyl Alcohol, Cinnamaldehyde and Cinnamic Acid in Bioconversion solution by Multiorder Ultraviolet Derivative Spectrometry

1,su guijiao1,Li Mahuang qiurong1,yu chang1, 1   

  • Received:2019-05-08 Revised:2020-02-20 Online:2020-05-25 Published:2020-05-15
  • Contact: Li Ma E-mail:gxumali@126.com

Abstract: To screen efficiently microorganism strain capable of biotransforming cinnamaldehyde into cinnamic alcohol, a new method of multi-order ultraviolet derivative spectrometry for the rapid determination of cinnamic alcohol, cinnamaldehyde and cinnamic acid in biotransformation liquid was established. The second-derivative peak is at 320nm and 306nm for cinnamaldehyde and cinnamic acid respectively and the third-derivative peak is at 256nm for cinnamic alcohol. At the fixed wavelength, the second-order derivative value of cinnamaldehyde and cinnamic acid had a good linear relationship with their mass concentrations in 0.52~9.27 mg/L and 0.51~9.24 mg/L, respectively, and the average recovery rate was 103.3% and 104.1%, RSD was 4.1% and 2.1%, respectively. The third-order derivative value of cinnamic alcohol had a good linear relationship with its mass concentration in 0.55~5.47 mg/L, and the average recovery rate is 98.0%, RSD was 3.5%.The measurement can be carried out on raw diluent samples, without extraction separation and can be used for rapid screening the microorganism strains that can biotransform biotransforming cinnamaldehyde into cinnamic alcohol.

Key words: ultraviolet derivative spectrometry, cinnamaldehyde, cinnamic alcohol, cinnamic acid, biotransformation

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