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• Bioengineering •     Next Articles

Apoptosis of Saccharomyces cerevisiae Induced by ε-Poly-L-lysine Combined with Reactive Oxygen Species

2, 2, 2, 2   

  • Received:2019-04-23 Revised:2020-03-27 Online:2020-05-25 Published:2020-05-15

Abstract: ε-Poly-L-lysine(ε-PL) is a green and safe natural preservative with broad spectrum antimicrobial activity. In this paper, Saccharomyces cerevisiae was used as a model fungus to study the changes of cell growth, morphology and reactive oxygen species(ROS) under the combined effects of different concentrations of ε-PL and its exogenous antioxidant N-acetylcysteine(NAC). Meanwhile, the bacteriostatic activity of ε-PL on Saccharomyces cerevisiae was explored. The results showed that cells treated with sublethal concentration were not smooth, the content of ROS was increased. Fluorescence microscopy observation and display results of phosphatidylserine externalization, chromatin pyknosis, mitochondrial transmembrane potential dissipation and DNA damage, which entered the early stage of apoptosis. Cells treated with lethal concentration were rough and pitted, the content of ROS was decreased, and fluorescence characteristics were obvious. Cells entered the late stage of apoptosis until death. After adding NAC, the ROS was decreased and the fluorescence characteristics was decreased. Therefore, it is suggested that the increase of ROS induced by ε-PL can lead cells to apoptotic stage and inhibit cell activity. In addition, NAC can reduce the antimicrobial activity of ε-PL.

Key words: ε-Poly-L-lysine(ε-PL), Saccharomyces cerevisiae, reactive oxygen species(ROS), N-acetylcysteine(NAC), apoptosis

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