Positive effects on the bioaccessibility of four bamboo flavonoids by pectin addition during simulated in vitro gastrointestinal digestion

Abstract

Abstract: Objectives: It is essential to increase the bioaccessibility of bamboo flavonoids during the gastrointestinal digestion in order to improve health benefits. The propose of this work was to clarify the protective effects of pectin for bamboo leaf flavonoid C-glycosides during simulated in vitro gastrointestinal digestion process. Methods: In this study, simulated in vitro gastrointestinal digestion models were used to determine the bioaccessibility of bamboo leaf flavonoid C-glycosides combined with UPLC analysis. Results: Direct digestion (without pectin) produced a significant decrease in flavonoid C-glycosides contents of extracts (P < 0.05) [收稿日期：2019-05-27 基金项目：浙江省淡水水产研究所开放课题（ZJK201912）；浙江省自然科学基金（LQ17C200002）；湖州市公益性研究项目（2018GZ28）第一作者简介：俞玥（1987—）（ORCID: 0000-0002-3369-1800），女，讲师，博士，研究方向为食品加工与安全。E-mail: yuyueoffice2012@126.com *通信作者简介：周冬仁（1980—）（ORCID: 0000-0002-0080-6982），男，高级工程师，副高，硕士，研究方向为渔业健康与食品分析。E-mail: 872559718@qq.com ]while pectin treatment counteracted the decrease significantly (P < 0.05). Moreover, the oral phase, gastric phase, and intestinal phase digestion were significantly different, and intestinal phase digestion obtained the highest decrease of the flavonoid C-glycosides contents (P < 0.05). It was highlighted that the bioaccessibility of flavonoids was significantly improved by pectin treatment (P < 0.05). Especially, bioaccessibility of isovitexin and vitexin were increased to 175.1% and 180.2%, respectively. Moreover, principal components analysis and cluster analysis were performed to describe the effection of the pectin addition for the bioaccessibility of the bamboo leaf flavonoids. for presenting the treatments. Conclusion: Altogether, these findings have stressed that pectin was useful to enhance the bamboo leaf flavonoid C-glycosides bioaccessibility indicating the promising applications for functional products development.

Key words: Bamboo leaf flavonoid, pectin, UPLC, in vitro gastrointestinal digestion, cluster analysis

CLC Number:

TS201.2

Zhan-Ming Li yue yu Huang DAI Lei Xu Dong-Ren ZHOU. Positive effects on the bioaccessibility of four bamboo flavonoids by pectin addition during simulated in vitro gastrointestinal digestion[J]. FOOD SCIENCE, 0, (): 0-0.

References

[1] GENTILE, D., FORNAI, M., PELLEGRINI, C., et al., Dietary flavonoids as a potential intervention to improve redox balance in obesity and related co-morbidities: a review[J], Nutrition Research Reviews, 2018, 31, 239-247. https://doi.org/10.1017/S0954422418000082.
[2] MA, Y., HE, Y., YIN, T., et al., Metabolism of phenolic compounds in LPS-stimulated Raw264. 7 cells can impact their anti-inflammatory efficacy: Indication of hesperetin[J], Journal of Agricultural and Food Chemistry, 2018, 66, 6042-6052. https://doi.org/10.1021/acs.jafc.7b04464.
[3] ZHAO, Z., WU, M., ZHAN, Y., et al., Characterization and purification of anthocyanins from black peanut (Arachis hypogaea L.) skin by combined column chromatography[J], Journal of Chromatography A, 2017, 1519, 74-82. https://doi.org/10.1016/j.chroma. 2017.08.078.
[4] ELEGBEDE, J.L., LI, M., JONES, O.G., et al., Interactions Between Flavonoid-Rich Extracts and Sodium Caseinate Modulate Protein Functionality and Flavonoid Bioaccessibility in Model Food Systems[J], Journal of Food Science, 2018, 83, 1229-1236. DOI: 10.1111/1750-3841.14132.
[5] PEREZ-MORAL, N., SAHA, S., PHILO, M., et al., Comparative bio-accessibility, bioavailability and bioequivalence of quercetin, apigenin, glucoraphanin and carotenoids from freeze-dried vegetables incorporated into a baked snack versus minimally processed vegetables: Evidence from in vitro models and a human bioavailability study[J], Journal of Functional Foods, 2018, 48, 410-419. https://doi.org/10.1016/j.jff.2018.07.035.
[6] MANDALARI, G., VARDAKOU, M., FAULKS, R., et al., Food matrix effects of polyphenol bioaccessibility from almond skin during simulated human digestion[J], Nutrients, 2016, 8, 568. https://doi.org/10.3390/nu8090568.
[7] RIBAS-AGUSTí, A., MARTíN-BELLOSO, O., SOLIVA-FORTUNY, R., et al., Food processing strategies to enhance phenolic compounds bioaccessibility and bioavailability in plant-based foods[J], Critical Reviews in Food Science and Nutrition, 2018, 58, 2531-2548. DOI: 10.1080/10408398.2017.1331200.
[8] XIANG, D., FAN, L., HOU, X., et al., Uptake and transport mechanism of dihydromyricetin across human intestinal Caco-2 cells[J], Journal of Food Science, 2018, 83, 1941-1947. DOI: 10.1111/1750-3841.14112.
[9] MARTíNEZ-LAS HERAS, R., PINAZO, A., HEREDIA, A., et al., Evaluation studies of persimmon plant (Diospyros kaki) for physiological benefits and bioaccessibility of antioxidants by in vitro simulated gastrointestinal digestion[J], Food Chemistry, 2017, 214, 478-485. https://doi.org/10.1016/j.foodchem.2016.07.104.
[10] GONZáLEZ-SARRíAS, A., ESPíN, J.C., TOMáS-BARBERáN, F.A., Non-extractable polyphenols produce gut microbiota metabolites that persist in circulation and show anti-inflammatory and free radical-scavenging effects[J], Trends in Food Science and Technology, 2017, 69, 281-288. https://doi.org/10.1016/j.tifs.2017.07.010.
[11] QUIRóS-SAUCEDA, A.E., PALAFOX-CARLOS, H., SáYAGO-AYERDI, S.G., et al., Dietary fiber and phenolic compounds as functional ingredients: interaction and possible effect after ingestion[J], Food & Function, 2014, 5, 1063-1072. DOI: 10.1039/C4FO00073K.
[12] VIUDA-MARTOS, M., LUCAS-GONZALEZ, R., BALLESTER-COSTA, C., et al., Evaluation of protective effect of different dietary fibers on polyphenolic profile stability of maqui berry (Aristotelia chilensis (Molina) Stuntz) during in vitro gastrointestinal digestion[J], Food & Function, 2018, 9, 573-584. DOI: 10.1039/C7FO01671A.
[13] 俞玥, 蔡泓历, 毛豪等. 黄原胶对体外模拟胃肠道消化过程中四种竹叶黄酮生物可及性的影响[J], 食品科学, 2019. http://kns.cnki.net/kcms/detail/11.2206.TS.20181214.1353.046.html
[14] AYYASH, M., JOHNSON, S.K., LIU, S.-Q., et al., In vitro investigation of bioactivities of solid-state fermented lupin, quinoa and wheat using Lactobacillus spp[J], Food Chemistry, 2019, 275, 50-58. https://doi.org/10.1016/j.foodchem.2018.09.031.
[15] AYYASH, M., AL-NUAIMI, A.K., AL-MAHADIN, S., et al., In vitro investigation of anticancer and ACE-inhibiting activity, α-amylase and α-glucosidase inhibition, and antioxidant activity of camel milk fermented with camel milk probiotic: A comparative study with fermented bovine milk[J], Food Chemistry, 2018, 239, 588-597. https://doi.org/10.1016/j.foodchem.2017.06.149.
[16] FARIDI ESFANJANI, A., ASSADPOUR, E., JAFARI, S.M., Improving the bioavailability of phenolic compounds by loading them within lipid-based nanocarriers[J], Trends in Food Science and Technology, 2018, 76, 56-66. https://doi.org/10.1016/j.tifs.2018.04.002.
[17] TOMAS, M., BEEKWILDER, J., HALL, R.D., et al., Industrial processing versus home processing of tomato sauce: Effects on phenolics, flavonoids and in vitro bioaccessibility of antioxidants[J], Food Chemistry, 2017, 220, 51-58. https://doi.org/10.1016/j.foodchem.2016.09.201.
[18] GAYOSO, L., CLAERBOUT, A., CALVO, M.I., et al., Bioaccessibility of rutin, caffeic acid and rosmarinic acid: Influence of the in vitro gastrointestinal digestion models[J], Journal of Functional Foods[J], 2016, 26, 428-438. https://doi.org/10.1016/j.jff.2016.08.003.
[19] YU, Y., LI, Z., CAO, G., et al., Bamboo leaf flavonoids extracts alleviate oxidative stress in HepG2 cells via naturally modulating reactive oxygen species production and Nrf2-mediated antioxidant defense responses[J], Journal of Food Science, 2019. DOI: 10.1111/1750-3841.14609.
[20] ZHANG, Y., JIAO, J., LIU, C., et al., Isolation and purification of four flavone C-glycosides from antioxidant of bamboo leaves by macroporous resin column chromatography and preparative high-performance liquid chromatography[J], Food Chemistry, 2008, 107, 1326-1336. https://doi.org/10.1016/j.foodchem.2007.09.037.
[21] WU, D., CHEN, J., LU, B., et al., Application of near infrared spectroscopy for the rapid determination of antioxidant activity of bamboo leaf extract[J], Food Chemistry, 2012, 135, 2147-2156. https://doi.org/10.1016/j.foodchem.2012.07.011.
[22] MATERSKA, M., Flavone C-glycosides from Capsicum annuum L.: relationships between antioxidant activity and lipophilicity[J], European Food Research Technology, 2015, 240, 549-557. https://doi.org/10.1007/s00217-014-2353-2.
[23] CHEN, G., LI, C., WANG, S., et al., Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures[J], Food Chemistry, 2019, 292, 281-293. https://doi.org/10.1016/j.foodchem.2019.04.060.
[24] CHEN, L., WU, J.e., LI, Z., et al., Metabolomic analysis of energy regulated germination and sprouting of organic mung bean (Vigna radiata) using NMR spectroscopy[J], Food Chemistry, 2019, 286, 87-97. https://doi.org/10.1016/j.foodchem.2019.01.183.
[25] CHEN, L., TAN, G.J.T., PANG, X., et al., Energy Regulated Nutritive and Antioxidant Properties during the Germination and Sprouting of Broccoli Sprouts (Brassica oleracea var. italica) [J], Journal of Agricultural and Food Chemistry, 2018, 66, 6975-6985. 10.1021/acs.jafc.8b00466.
[26] GONZáLEZ-MONTOYA, M., HERNáNDEZ-LEDESMA, B., SILVáN, J.M., et al., Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation[J], Food Chemistry, 2018, 242, 75-82. https://doi.org/10.1016/j.foodchem.2017.09.035.
[27] PETRY, F.C., MERCADANTE, A.Z., Impact of in vitro digestion phases on the stability and bioaccessibility of carotenoids and their esters in mandarin pulps[J], Food & Function, 2017, 8, 3951-3963. DOI: 10.1039/C7FO01075C.
[28] PALMERO, P., PANOZZO, A., COLLE, I., et al., Role of structural barriers for carotenoid bioaccessibility upon high pressure homogenization[J], Food Chemistry, 2016, 199, 423-432. https://doi.org/10.1016/j.foodchem.2015.12.062.
[29] KERMANI, Z.J., SHPIGELMAN, A., PHAM, H.T.T., et al., Functional properties of citric acid extracted mango peel pectin as related to its chemical structure[J], Food Hydrocolloids, 2015, 44, 424-434. https://doi.org/10.1016/j.foodhyd.2014.10.018.
[30] ALBA, K., KONTOGIORGOS, V., Pectin at the oil-water interface: Relationship of molecular composition and structure to functionality[J], Food Hydrocolloids, 2017, 68, 211-218. https://doi.org/10.1016/j.foodhyd.2016.07.026.
[31] KASTNER, H., EINHORN-STOLL, U., DRUSCH, S., Structure formation in sugar containing pectin gels-Influence of gel composition and cooling rate on the gelation of non-amidated and amidated low-methoxylated pectin[J], Food Hydrocolloids, 2017, 73, 13-20. https://doi.org/10.1016/j.foodhyd.2017.06.023.
[32] WANG, Q., REN, Y., DING, Y., et al., The influence of pH and enzyme cross-linking on protein delivery properties of WPI-beet pectin complexes[J], Food Research International, 2018, 105, 678-685. https://doi.org/10.1016/j.foodres.2017.11.076.
[33] CHEN, H., YAO, Y., Phytoglycogen improves the water solubility and Caco-2 monolayer permeation of quercetin[J], Food Chemistry, 2017, 221, 248-257. https://doi.org/10.1016/j.foodchem.2016.10.064.
[34] JO, M., BAN, C., GOH, K.K., et al., Gastrointestinal digestion and stability of submicron-sized emulsions stabilized using waxy maize starch crystals[J], Food Hydrocolloids, 2018, 84, 343-352. https://doi.org/10.1016/j.foodhyd.2018.06.026.
[35] JAKOBEK, L., Interactions of polyphenols with carbohydrates, lipids and proteins[J], Food Chemistry, 2015, 175, 556-567. https://doi.org/10.1016/j.foodchem.2014.12.013.