Application of Real-Time PCR Assay in the Identification of Caprinae Meat

doi:10.7506/spkx1002-6630-20190219-104

Abstract

Abstract: A real-time polymerase chain reaction (real-time PCR) method for the identification of meat from Caprinae (including goats and sheep) was established and optimized based on the inter-species difference of cytochrome b (cyt b) gene. We evaluated the specificity, sensitivity, amplification efficiency, detection limit, reagent consistency and applicability of this method, and we invited five domestic authoritative institutions to validate it. The results showed that this method could be suitable for detecting Caprinae meat products with different substrates because of its good repeatability, wide applicability, high amplification efficiency (> 95%) and accuracy (100%).

Key words: species identification, Caprinae, method investigation, practical application

CLC Number:

TS251.7

SHANG Ke, LIANG Hengxing, ZHANG Biao, DUAN Qingzi, WANG Wei, ZHANG Yu. Application of Real-Time PCR Assay in the Identification of Caprinae Meat[J]. FOOD SCIENCE, 2020, 41(12): 318-325.

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Application of Real-Time PCR Assay in the Identification of Caprinae Meat

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