FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (12): 326-331.doi: 10.7506/spkx1002-6630-20190225-180

• Safety Detection • Previous Articles    

Evaluation of Commercial Rapid Test Strips for Detection of Aflatoxin B1 in Edible Oil

ZHANG Wei, ZHANG Wenzhong, GUO Ping, HU Zhongyi, Lü Xiaoli   

  1. (Jiangxi Institute for Food Control, Nanchang 330001, China)
  • Received:2020-06-24 Revised:2020-06-24 Online:2020-06-25 Published:2020-06-22

Abstract: To evaluate and test three rapid aflatoxin B1 (AFB1) test strips from different manufacturers, blind samples were prepared by blank matrix addition, and their uniformity and stability were verified in this study. We investigated the effects of AFB1 concentration and the number of samples on performance indicators of the test strips. The results showed that the sensitivities of the three products were 83%, 100% and 100%, respectively, the specificities were 76.2%, 79.3% and 67.3%, respectively, the false negative rates were 17%, 0% and 0%, respectively, the false positive rates were 23.8%, 20.7% and 32.7%, respectively, and the relative accuracies were 78.9%, 87.6% and 80.6%, respectively. We conducted in-depth discussions on AFB1 concentration setting, stability evaluation and result judgment, and clarified that there were many factors affecting the evaluation of these products. This study is expected to be helpful for the evaluation of rapid test products.

Key words: aflatoxin B1, food safety rapid test, colloidal gold immunochromatographic test strip, evaluation

CLC Number: