Abstract: An efficient, green and fastdynamic matrix solid phase dispersion ionic liquid-based aqueous two-phase microextraction method was developed and applied to the determination of five triazine herbicides in grain crops, including simmatone, atrazine, tertinone, tertinin and dipropetryn. The target analytes were separated and determined by high-performance liquid chromatography. In this method, the ionic liquid solution was used as the extraction solvent. The matrix solid phase dispersion method was used as a pretreatment technique. The ionic liquid solution was used to elute the target analytes from the extraction column. When sodium dodecyl benzene sulfonate was added to the collected eluate, it self-aggregated with the ionic liquid to form an aqueous two-phase system. The target analytes were enriched in the upper phase solution. The Plackett–Burman (P-B) design was used to judge the significance of various factors on the response value and the Box-Behnken Design (BBD) was used to optimize the response surface of the significant factors. The results demonstrated that there was a good linear relationship (r≥ 0.9969) of the target analytes within the linear range of 10.00~100.00 μg/kg. The limits of detection for the analytes were 0.62~0.76 μg/kg and the limits of quantification were 2.06~2.53 μg/kg. The spiked recoveries were 82.92%~106.98%. The present method has simple operation, shorter extraction tim, low consumption of organic reagents, environment friendly. As a result, it can be used for the analysis and detection of triazine herbicides in grain crops.

Key words: Matrix solid phase dispersion, aqueous two-phase extraction, triazine herbicides, ionic liquid, grain crops, high performance liquid chromatography