Abstract:

A molecular imprinted monolithic column was prepared for providing stationary phase for the HPLC analysis of melamine in dairy products, in which the mobile phase was a mixture of 10% aqueous methanol solution and 0.1% aqueous acetic acid solution (5:95, V/V) at a flow rate of 0.5 mL/min and the detection wavelength was set at 240 nm. There was a good linear relationship between HPLC peak area and melamine concentration over the range from 1.0 to 100.0 μg/mL (r = 0.9987). Average recoveries for melamine in a blank sample spiked at 3 levels were between 87.0% and 100.5%, with a RSD of less than 5% (n = 6). In conclusion, this method is easily operated, highly selective and has no requirement of complicated sample pretreatment and is applicable for the determination of melamine residue in dairy products.

Key words: molecular imprinting technique (MIT), high performance liquid chromatography (HPLC), melamine, dairy products