%0 Journal Article %A WANG Yan %A CHENG Hui-juan* %A ZHU Ling-ling %A SUN Zhen-xin %A LI Lu-tian %T Effect of Sodium Houttuyfonate on Pseudomonas aeruginosa Biofilm %D 2013 %R 10.7506/spkx1002-6630-201311038 %J FOOD SCIENCE %P 173-176 %V 34 %N 11 %X
Purpose: To investigate the effect of sodium houttuyfonate on the formation of Pseudomonas aeruginosa biofilm at different stages. Methods: MTT assay was used to detect the antibacterial and metabolic effect of sodium houttuyfonate at different concentrations on Pseudomonas aeruginosa. An electron microscope was used to observe the inhibitory effect of sodium houttuyfonate on the formation of Pseudomonas aeruginosa biofilm at different stages. Viable cell count on filter membrane was used to evaluate the inhibitory effect of sodium houttuyfonate on the adhesion of Pseudomonas aeruginosa biofilm. Results: Minimum inhibitory concentration was 300 μg/mL, and the antibacterial activity of sodium houttuyfonate was positively dependent on its concentration. The concentration necessary for 50% and 80% inhibition of biofilm formation were 9.37-37.5 μg/mL and 37.5-150 μg/mL, respectively, and as a result, biofilm bacterial populations were reduced significantly compared with normal control group (P < 0.05). Scanning electron microscopic (SEM) observation revealed that bacteria in sodium houttuyfonate group were in a planktonic state with smooth surface and no mucus-like substances, and the number of viable cells on carrier in the membrane filter was apparently smallers than that of normal control group. Sodium houttuyfonate at high dose had significant differences from control (P < 0.05) in adhesion, initial formation of biofilm and the formation of mature biofilm. Conclusion: As an active ingredient in Houttuynia cordata, sodium houttuyfonate has antibacterial activity against Pseudomonas aeruginosa, and can effectively inhibit the formation of biofilm.
%U https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201311038