%0 Journal Article %A XIA Huili %A CHEN Sisi %A CHEN Xiong %A DAI Jun %A HUANG Yanan %A XIE Ting %A LI Ailing %A WANG Zhi %T Identification of Clostridium butyricum and Optimization of Fermentation Medium for Its Growth %D 2017 %R 10.7506/spkx1002-6630-201708010 %J FOOD SCIENCE %P 56-62 %V 38 %N 8 %X An anaerobic Clostridium strain designated HBUT-01was screened from river sludge. By 16S rDNA sequence analysis, the strain was identified as Clostridium butyricum and its 16S rDNA fragment shared 99% similarity with that of C. butyricum DSM 10702T (AQQF01000149). The response surface methodology (RSM) was used to optimize the fermentation medium for this isolated strain, and a quadratic regression model with yeast exact, CaCO3 and glucose as independent variables was established by Design-Expert 8.0 software. The optimum medium was determined as follows: glucose 1.60%, yeast extract 0.72%, peptone 2.50%, CaCO3 0.43%, K2HPO4 0.05%, MgSO4·7H2O 0.08%, and MnSO4·H2O 0.002%. Batch cultures were carried out in a 10-L bioreactor, and an increase in specific productivity of butyrate (qp) of 20% and an increase in biomass yield coefficient (Yx/s) of 132% were obtained as compared with that before optimization. The results suggested that the impact of acid stress on the cells was relieved effectively and cell activity was also maintained with the optimized medium, which resulted in an increased biomass, reaching 9.32×108 CFU/mL at 14 h, which was increased by 2.24 times as compared to that with the basic medium. %U https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201708010