The activities of four enzyme (γ-glutamyl transpeptidase, γ-GT; alkaline phosphatase, AKP; peroxidase, LP; amylase, AMY) in Henan dairy goat milk (a local variety of dairy goat in the west part of Henan province) was measured and the milk protein composition was determined by SDS-PAGE in this study. The results showed that the activities of r-GT, AKP, LP and AMY in colostrum and common milk of Henan dairy goat were 322.46 U/100 mL and 247.71 U/100 mL, 248.62 U/100 mL and 200.14 U/100 mL, 281.76 U/mL and 205.07 U/mL, and 71.20 U/100 mL and 22.15 U/100 mL, respectively. The activity of AKP was significantly higher in colostrums milk compared to common milk (0.01