A single crystal of curcumin was obtained from the turmeric of Shuangliu County of Sichuan Province, China by usingseparation and purification technique of solvent extraction, column chromatography and recrystallization etc. The molecular andcrystal structure of curcumin were identified by IR, UV, MS, 1HNMR, 13CNMR and X-ray single crystal diffraction. Curcumincrystal belongs to monoclinic with P2/n space group. The unit cell data are: a = 1.2695(3)nm, b = 0.72075(16) nm, c=1.9960(4) nm,α=90°β=95.098°γ=90°. The molecule of curcumin damonstrates a nonlinear keto-enol tautomerism structure andthere are four curcumin molecules in each cell.

 This experiment was conducted for further investigation into m-calpain activity and its role in beef aging. Fresh yellowcattle spleen (within 30min postmortem) was obtained and treated with procedures such as ultrahigh refrigeration homogenization,ionic exchange chromatography, hydrophobic chromatography, etc. The resulted m-calpain with specific activity 39.7U/mg wasverified by way of SDS-PAGE .

 The antioxidative activity of tea polysaccharide was determined by the capacity of scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical, and the evaluated unit was expressed as the equivalent concentration of ascorbic acid. Afterextraction, the antioxidative activities of tea polysaccharides from various teas and different extraction processes were determined.The results showed that there are remarkable effects of region, breed and extraction process on the capacity of scavenging DPPHfreer adical.

This paper studied the oxidative stability of Conjugated Linoleic Acid-Ethyl Ester (CLA-EE) and Conjugated LinoleicAcid Tyiglycerides(CLA-TG), and compared it with the safflower seed oil which was of rich unsaturated fatty acid. This paperalso studied the effects of transitional metallic ions and antioxidants on the oxidative stability of CLA-EE and CLA-TG. The resultsshowed that the oxidative stability of CLA-TG was higher than CLA-EE, their oxidative stability could be improved when theamount of vitamine E was added 0.02%. Nevertheless the addition of 0.02% FeCl3 could decrease their oxidative stability, whereasthe addition of vitamine C had no enhancement on the antioxidative effect of vitamine E in the oil system of Conjugated LinoleicAcid esters.

 Some changes of pigment content, protein content, total lipid content and ability of eliminating free radical ofChlamydomonas nivalis exposed to different dosage ultraviolet radiation have been studied. The results showed that Chlamydomo-nas nivalis had quite strong capability to resist ultraviolet radiation. There grew more chlorophyll and carotenoid contents in thecell in accordance with the length of radiation time. And remarkable increase of astaxanthin content after being irradiated has alsobeen discovered. Basic biochemical components of Chlamydomonas nivalis have been obviously changed after the treatment suchas the content of protein decreased by 18.3%, while the total lipid content on the contrary increased by 32.0%, and the capabilityof eliminating free radicals of Chlamydomonas nivalis in organic solvent has also been enhanced.

The structure properties of the adzuki bean starch have been studied. The range of starch granule size is 18～80μmand its mean granules diameter is 40.8μm. Obvious birefringence been observed. Adzuki bean starch is attributed to A-type crystalstructure and its degree of crystallinity is 40.5%. The compound of adzuki bean starch with I2 has the maximal absorbance at the618nm and its amylose content is 33.2%. The swelling ability and solubility of the adzuki bean starch is better than maize butnot like that of cassava. Adzuki bean starch paste belongs to pseudoplastic fluid. The shear resistance and the setback capacityare stronger than maize and cassava. Adzuki bean starch has good hot and cold paste stability. Its monoglyceride has specific impacton adzuki bean starch paste.

Acidity change and apparent viscidity change of yogurt mixed with different amount (5%,7.5% and 10%) and differenthydrolytic degree (3.12%,5.77% and 8.83%) pea-protein hydrolysate by Flavourzyme were studied in this paper. Rheologicalshear stress, parameter and viscosity index in different shear rate were studied in yogurt.The result showed that it took shortesttime to be the fermentive and for yogurt when milk was mixed with 7.5%～10% enzymatic soy-protein hydrolysate whichhydrolytic degree was 8.83%. The apparent viscidity of fermented yogurt was increased when milk was mixed with 10%DH 3.12% enzymatic soy-protein hydrolysate. Flexibility of yogurt was reduced when milk mixed with soybean hydrolysateand the yogurt was non-newtonian fluid. Shear stress of yogurt in high shear rate was far higher than CK when milk was mixedwith DH 3.12% enzymatic soy-protein hydrolysate.

The rheological property of carrot nectar was studied in this paper. It is found that the apparent viscosity of carrotnectar increases with the increasing of the pulp content or the decreasing of the temperature, also the increasing of homogenizationpressure, and the increasing first and then decreasing of the apparent viscosity when the homogenization pressure is higher than20MPa.

The antioxidant properties of water extracts of oolong tea (Camellia sinensis, commercial name: Fenghuangdancong),green tea (Longjing and Biluochun) and black tea (Dianhong and Yingdehongcha) were investigated. The oolong tea have high wateryield, concentration of amino acid and soluble sugar. Similar to green tea, oolong tea extracts had a strong antioxidant property(higher radical scavenging activity and lower peroxide value), which was attributed to a high concentration of polyphenols,particularly in catechins and theaflavin. Furthermore, the antioxidant activity of oolong tea was related to catechin concentrations,but not to total polyphenols.

In this study, 27 strains of lactic acid bacteria were isolated and identified from 16 samples of traditional indigenousdairy products collected from Inner Mongolia, including 4 strains of Lactobacillus casei subsp. Pseudoplantarum, 1 strain ofLactobacillus casei, 1 strain of Lactobacillus pentosus, 1 strain of Lactobacillus arabinosus, 1 strain of Enterococcus faecalis, 2strains of Enterococcus faeclum, 12 strains of Lactococcus lactic. subsp. Lactic, 2 strains of Lactococcus garvieae and 3 strainsof Streptococcus ferus. Each strain was used to ferment 10%(W/V) skim milk. The average acidity is 52.3°T. The specitic acidityof IML15-1 of the Lactobacillus is 101.8°T and its viscidity 1307.5mPa·s. And the viscidity of IMS8-2 of the Lactococcireaches 1567.5mPa·s.

 Calpain 3 is a skeletal muscle-specific calpain in calpain family. Isolation of the intact 94kDa enzyme has been difficultto achieve due to its rapid autolysis, and uncertainty has arisen over its dependence for Ca2+ activity.In the study, calpain3 crudeextract was obtiained, and found that in the presence of Ca2+, calpain3 cleaved itself into several fragments. This indicated thatthe autolysis of calpain3 was dependent on the concentration of Ca2+. It was also found that the autolysis of calpain3 proceedsin four consecutives steps: 89, 68and 58kDa fragments are produced as intermediates before a stable 55kDa fragment appears.

The subunits component and 7S/11S ratio of the protein extracted from different soy species were studied by thediscontinuous lauryl sodium sulfate-polyacrylamide gel electrophoresis techniques. The results showed that the species varietyaffected the component of protein subunits apparently. The physical properties of soy protein, hardness, elasticity and EAI(emulsifying ability index), were measured by rheometer and spectrometer. After a statistical correlation analysis between thecomponent of protein subunits and the three indexes was made, it was found that the significance was varied with differentsubunits and 7S/11S ratio played a significant role to the physical properties. The research of protein structure of the differentspecies would be useful and helpful to study the physical properties of the soy protein.

 Culturing Porphyridium spp. with optimized culture medium and simple photobioreactor was investigated. Apractical technical process on direct separation of Polysaccharide of Porphyridium(PSP) from broth was established, and PSPwas primarily analyzed. The results showed that the biomass of cultured Porphyridium reached 2.1g dry weight per litre, yieldof primary PSP 0.378g/L and efficiency of ion exchange about 78%. PSP was basically composed of xylose, glucose and galactose,with a mass ratio 13.68:7.83:5.23 respectively. The result by IR analysis indicates that PSP has its characteristic absorption peak,and its hexose is of pyranoid form while PSP is sulfated polysaccharide, and its content of –SO3- is about 4%.

The result indicated that the fructosyltransferase from a wild strain of Aspergillus sp.JN19 was a intracellular enzyme.The optimum temperature and pH for the high activity of fructosyltransferase were 50℃ and 5.5, respectively. Mg2+ ions enhancedfructosyltransferase activity by 2.2-fold whereas Cu2+ ions completely inhibited enzyme activity. The enzyme synthesized newfructosyl oligosacchrides by transfering fructose moiety of sucrose to various acceptors.

The method of extracting and purifying polysaccharide from Momordica charantia was suggested in this paper. Itsphysical and chemical characteristics and biological activity were determined. The polysaccharide was extracted from Momordicacharantia by adding two species of enzymes. The optimum hydrolysis conditions were got. The polysaccharide was furtherpurified with DEAE-32 column chromatography and Sephadex G-75 column chromatography respectively until the single peakwas got, and named as PMCⅠ. The acidic hydrolysis solution of PMCⅠwas analysed by means of TLC: its constituentsrespectively glucose, galactose, mannose, rhamnose. The study for its biological activity showed that it could eliminate free radicalssuch as ·OH and O2·, and its IC50 for ·OH and O2· were respectively 0.0017mg/ml and 0.005mg/ml. So it showed theactivity of antioxidant. The experiments of inhibiting germs show that PMCⅠcan inhibit germs such as S.Aureus, E.coli, B.subtilusand S.typhimurium. The IC50 for those were respectively 0.10, 0.15, 0.13, and 0.13mg/ml, the most remarkable of which was S.Aureus.

The effects of different extract conditions on acetylcholinesterase(AChE) inhibitory activities of Douchi werestudied. The in vitro AChE inhibitory activities of 9 kinds of commercial Douchi were assayed, expessed as IC50. Ethanol extractsshowed highest AChE inhibitory activity with IC50 value as 0.023mg/ml, while water extracts did not show any inhibition. Whenextracted by ethanol, the IC50 of Douchi inceased with the decrease of solid/liquid rate and ethanol concentration. No significantdifferences were observed within IC50 values of the Douchi extracts with different extract time. 80% ethanol extracts of these nineDouchi inhibited human acetylcholinesterase with IC50 values ranged from 0.033 to 1.110mg/ml. This novel finding suggests thatDouchi containing anti-acetylcholinesterase components, might be used to retard the progression of Alzheimer disease, andindicates the potential to be developod into new functional foods.

The antioxidation effects of green tea, black tea and wulong tea were studied by the flow-injection chemiluminescence.The results indicated that natural tea, black tea and wulong tea effectively restrain the chemiluminescence of luminol induced bysuper oxygen anion free radical. The strength of the chemiluminescence presents the descending trend with the increasing of theconcentration of green tea, black tea and wulong tea. A comparison of the antioxidation effects shows that the strength is in theorder of green tea, black tea and wulong tea.

This article studied the extracting technology of the red pigment of Viburnum cylindricum Buch with HPD-100 resin.In the course of desorbing the absorbed red pigment with 80% ethanol .HPD-100 resin was selected for the experiment. After usingof 20 times, the absorption factor is still very stable.

Lactobacillus acidophilus La-w1 and La-w2 from different sources were successfully fused by using the protoplastfusion technique. Fusion hybrid La-F1 was obtained, and its growing characteristics cultivated in skim milk for 48 hours at 37℃ werestudied. Results showed that La-F1 has better acid and bile tolerance than initial strains and the fermented characterics meet therequirement of lactic acid fermented food.

During processing of Nanjing cured-dry duck, the changes of peptides and free-amino acid(FAA) have an importanteffects on its unique flavour. Cured-dry duck samples of different processing phases were used to study the changes of peptidesand free-amino acids by HPLC in this paper. The results showed that the level and amount of proteolysis increased with theprocessing time by traditional technology, while the amount of peptides was positively correlated with FAA contents(R2=0.86).

Rapeseed albumin was hydrolyzed in different steps by Alcalase and Flavourzyme. The degree of hydrolysis ofRapeseed Albumin could go up to 28% from 14.72%. The rapeseed peptide(RSP-R) was separated and purified by Sephadex G-25 gel filtration chromatography, so three parts of peptides were obtained: RSP-1 of molecular weight over 5kD, RSP-2 of molecularweight about 1050D and RSP-3 about 560D. As RSP-2 is the primary part of RSP-R, its protein and total sugars contents are79.23% and 13.88% respectively. All the results would provide ideal materials and theory foundation for further research.

 To degrease and to decolor on mongolia mushroom through supercritical CO2 fluid extraction, the effects of this methodon polysaccharide extraction were studied . The optimum conditions of supercritical CO2 fluid extraction were determinedthrough orthogonal test: the pressure 30 MPa, temperature 45℃, time 60min, CO2 flow 26L/h. The percentage of extraction is2.11%. The color of product is milk white, the flavor pure and the fat content 0.01%. By using this material to extract the mongoliamushroom polysaccharide, the extraction rate of polysaccharide is 6.24% and the polysaccharide’s color is milk white with nostrange smell. The extraction rate is 4.2 times and 1.8 times in comparison with non- supercritical CO2 fluid extraction andtreatment by solvent respectively.

The optimal extraction conditions of astaxanthin esters from freeze-dryed algae sample were: extracted with the mixedsolvents of ethyl acetate and ethanol, temperature 25℃, extracting time 6 h, ratio of ethyl acetate and ethanol 1:2, and proportionof solid to liquid 1:120 (g/ml). Astaxanthin esters could be hydrolyzed to free astaxanthin by saponification, while low temperaturewould help astaxanthin decrease disruption during the saponification. The saponification conditions were also studied, and theeffects of concentration of methanolic KOH solution and the saponification time were assayed respectively. The optimumconditions were 6ml of 0.06mol/L methanolic KOH solution added to 4ml pigment extract solution, and saponified 12 h at 4℃in darkness. With this method, (575.86±5.68)μg trans-astaxanthin is obtained from 100mg dry algal cells.

Response surface methodology (RSM) was used to optimize the medium components of B.breve A04. In the firststep of the optimization, a factional factorial design was used to evaluate the effects of nine factors. The results showed that threefactors, including glucose, yeast extract and initial pH had significant effects on bacteria dry weight. Secondly, the path ofsteepest ascent was used to gain the optimal region of the three factors. At last, the concentrations were optimized using CCRD(Central Composite Rotable Design) and RSM analysis. As a result, the contents were obtained as follows: (/L): glucose5.194g,yeast extract2.829g, FOS 2g, tomato juice50ml, liver infusion 5g, Tween80 1ml, cystein-HCl 0.3g, and pH6.463. Final dryweight of biomass was obtained as 4.462g/L, 1.43 higher than before 3.16 g/L.

Inulinase from Aspergillus ficuum SK004 was immobilized on cotton cloth activated with p-toluenesulfonyl chloride(tosyl chloride). The immobilization conditions were optimized and the results showed that the optimal amount of dry pyridinewas 10ml to each gram of cotton and the ratio of tosyl (g) /pyridine (ml) was 1g:1ml. The optimal coupling pH was found to be4.5 and ionic strength was 0.2mol/L. 80U enzyme was immobilized onto each gram of cotton cloth. The highest enzyme activityyield achieved 83.60% through orthogonal test. At these conditions, immobilized enzyme reacting with 250g/L inulin for 1.5h gained28.21% FOS (fructose oligosaccharide) analyzed by HPLC. Among different fibrous materials tested, the charpie cotton clothshowed the highest immobilized enzyme activity.

 Effects of different factors, such as acid-base properties, extraction temperature, time, power and the ratio of liquidto solid on extraction of red pigment of sorghum were comparatively investigated by three kinds of methods, i.e., traditional Soxhletextraction, microwave-assisted extraction and supersonic extraction. The experimental results demonstrated that the optimalconditions for Soxhlet method were 70%(V/V)ethanol, a 3.05 of pH value, ratio of liquid to solid of 100:1(ml:g), 75℃ of extractiontemperature and extraction time of 240 min, for microwave-assisted extraction were 70%(V/V) ethanol, a 2.0 of pH value, ratioof liquid to solid of 45:1(ml:g), 720 watt of power, and extraction time of 1.5 min, and for supersonic extrction were 70%(V/V)ethanol, 65℃ of extraction temperature, 80 watt of power, ratio of liquid to solid of 45:1(ml:g), extraction time of 60 min. Theyield rate of traditional Soxhlet, microwave-assisted and supersonic extraction are 16.5%, 15.03% and 13.38% respectively.Though the yield rate of traditional Soxhlet is higher than that of microwave-assisted extraction, the microwave-assisted methodhas many advantages, such as saving time, fast extraction, low energy consumption and solvent saving for synthesis, and simpleoperation processes.

Kefiran is a water soluble exopolysaccharide produced by lactic acid bacteria in kefir. The molecular weight is morethan 1000 kD. Water extraction and alcohol precipitation are used to isolate the kefiran from kefir. Factors influencing the extractionand the separation of extracellular kefiran and capsular kefiran in the fermented milk system are studied. The optimum separationconditions are: the fermented milk by adding 2% NaCl solution; 15% trichloroacetic acid ( based on the mass of the supernatant)to eliminate remnant protein; and alcohol for precipitation at pH 7.0. The kefiran yield is increased from 139.66mg/L to 427.33mg/L afteroptimization. And the optimum method to extract capsular kefiran is by sonication at 50W for 3min at 4℃.

The microecological stability of the freeze-dried powder of Bacillus natto was investigated. The results showed thatthe powder could resist acid, alkali, pepsin, trypsin and gallbladder salt, to assure that enough Bacillus natto could successfullypass and survive in the intestines and play their role of microecological activity. Moreover, the powder shows excellent stabilitiesduring room temperature storage for 120 days after 80℃ heat treatment for 60 minutes, However, it shows less stable after-20℃ storage.

 The effects of different metal ions on cell growth, cell morphology and astaxanthin biosynthesis of Phaffia rhodozymawere studied. It indicated that Mn2+ showed clear positive effects on the β-D-glucosidase activity and cell morphology in seedculture medium. The initial ferment medium containing 0.1g/L Mn2+ could improve obviously the cell growth and astaxanthinbiosynthesis. The optimal conditions obtained by orthogonal experiment were: 0.1g/L Mn2+, 0.7g/L Mg2+ and 0.2g/L Ca2+ in theferment medium. Under the optimal conditions, the cell grows rapidly, and the astaxanthin yield reaches 2.95mg/L at 57 h, whichis 1.31 times of that under no added metal ions (at 72 h).

The crude antihypertensive peptides extracted from wheat germ, were purified by chromatography on macroporousresin column. The recovery rate of antihypertensive peptides was defermined by ultraviolet absorbance spectrograde, whilechlorine content was determined by potassium chromate assay method. Total desalination rate calculated by ash content,nitrogen content assessed by KJedahl method and angiotensin converting enzyme inhibitor (ACEI) activity in vitro determinedby HPLC. Results: The desalination method was simple and feasible. The maximal disposal value of macroporous resin was 12.8mg/ml, chlorine remove rate 92.31%, the total desalination rate 90.14%, and recovery rate of antihypertensive peptides morethan 85%. In average, nitrogen content increased from 5.23% to 12.34%, and in vitro ACEI activity enhanced from 47.42% to70.40%.

In this paper, the litchi pulp affer preliminary storge, with conditions of open oxygen inoculating time, main fermentedtemperature , high content ascorbic acid content and polyphenol ,etc. to affect the litchi fruit wine oxidizesation. The results showed:(1)The litchi pulp added with 30% sugar and 250×10-6 Na2SO3 and stores -10～-20℃, and fermented, and there was notsignificant difference on oxidization degree from the fresh fruit or the litchi pulp to be stored in preliminarily. (2)it could reducethe fruit wine degree of oxidization in litchi fruit juice 36h with little oxygen inoculated and fermenation in 10～15℃ and: (3)Highcontents of ascorbic acid and polyphenol help resist oxidation in the litchi fruit wine.

Effect of temperature, proportion and time on extraction of the red dates polysaccharide were studied by orthogonalexperiment design and the optimum proportion A2B2C3 was chosen preliminarily. Meanwhile the author analyzed the componentof polysaccharide by infrared spectrum, ultraviolet spectrum and paper chromatography. The purification from the decoloration,removing proteins and others were deeply studied, the results showed that the red dates polysaccharide was a mono-ingredient,composed of D-Galactose and D-Xylose. The ways of active charcoal decoloration and proteins Sevag removing have significantefficiency.

In this paper, the optimum solvent for the extraction of natural edible red pigment from Jujube and the characteristicsof the pigment were studied. It was clear from the results that among the several chemical solvents tested for the extraction of thepigment, 50% ethanol (pH3) was the optimum solvent for the extraction of the pigment from jujube. The ODλmax of the jujubepigment was 320nm. The pigment was stable when it was tested by sunshine, temperature and most of the metal ions (Ca2+, Mg2+,Mn2+, Zn2+) respectively. Most of the metal ions could not interfere the stability of the pigment except Fe3+. Jujube pigmentwas stable under any pH condition, both in acid and alkaline. Results showed that the jujube pigment was worth of exploitingand utilizing. It could be used as one of the resources of natural edible pigments.

 Preparation technology of sulfated polysaccharides from Undaria Pinnatifida (Charv.) Suringer has been studied;one-factor experiment, one afactor experiment orthogonal experiment were investigated. The optimal condition chosen extractiontemperature 100℃, extraction time 4 h, ratio of solution to algae power 80. Follow this condition, we get three samples use grade-precipitation by alcohol, the average content of polysaccharides, sulfate, protein is 28.0%, 10.8%, 0.71% respectively. From IR,the characteristic peak of polysaccharides, sulfate can be seen, and sulfate link polysaccharides at C2 or C3 as equatorial, the samplesof polysaccharides are pyranose of β-glycosides mainly.

 For improving the quality of traditional Chinese roasted green tea, fresh leaf spreading, steaming to de-enzyme, anddehydrating for new-type roasted green tea process were studied. Compared with the traditional processing technique, this newprocessing technique of long-shape roasted green tea could not only improve the infusion color without smoking-smell, but alsoincreasing the amino acid content by 23.8% and total sensory test score up to 3.8. For the round-shape roasted green tea, thisnew technique could also improve the liquor color without smoking-smell, and increase the amino acid content by 5.2% andchlorophyll content up to 5.6 %.

The paper investigated the reasons of limit-exceeding problems of phosphate and for some methods to solve thisproblem. The results indicated that the total phosphate was considerable higher with in raw material. The results of spiced beefprocess indicates that by controlling 2‰ of phosphate, added and 75% product yield, there could be of no limit exceeding andgood quality product.

 Solid phase extraction(SPE)and gas chromatography mass spectrometric method were applied for the determinationof thiamphenicol residues in fish samples. The fish thiamphenicol was extracted by ethyl acetate under shaker .The ethyl acetateextracts were further purified and enriched by silica and C18 solid phase extraction cartridges. The final extracts were derivatisedand thiamphenicol was quantitatively determined by gas chromatography mass spectrometry in selected ion mode(ions selected409、411、499、501). The extraction and purification conditions were optimized. The recoveries of this method forfortified samples were between 80% ～ 95%, and the relative standard deviations were between 3.6%～8.1%.The detection limitof the method was below 0.3μg/kg.

A new kinetic spectrophotometric method for the determination of trace vanadium (V) has been developed. Themethod is based on the inhibitory effect on fading reaction of trace vanadium(V) on reducing of arsenanzo I (AsA I) with sodiumhypophosphite in 0.02mol/L dilute sulphuric acid solution. The detection limit is 0.052μg/L and the linear range of determinationis 0～4.0μg/L. The method has been applied to the determination of trace vanadium (V) in food samples with satisfactory results.

 Ethephon was applied widely to accelerate the ripening of fruit and vegetable as a plant-growth regulator. But at thesame time, it easily became the residue contamination in food. A simple and fast determination method of ethephon residue in foodwas therefore developed by means of the headspace gas chromatography. The sample and lye were first loaded in headspace bottle,then the headspace bottle was airproofed and heated up. The headspace gas of the sample was extracted and detected by GC withFID, and the quantitation was made by standard curve. The minimum detection limit was 0.01mg/kg, the average recovery 99.1%～105.0%, the relative standard deviation 8.64%～10.10%, and the linear range of method 0.01～100mg/kg. This determinationmethod is feasible to test the ethephon residue in fruits and vegetables.

The conditions were studied for assaying of acetylcholinesterase (AChE) activity from rabbit serum, as the targetenzyme of organophosphate and carbamate pesticide. The results showed that the optimal reaction conditions were: 3.0ml of pH8.0buffer solution of phosphoric acid, 50μl of 0.6% DTNB and 100μl of 0.75% AChE were mixed with 50μl of 1% BTCI at 35℃,and then value detected of OD412 after 180s. The AChE activity showed good inhibition effect on the prothiofos, anorganophosphate pesticide, with 0.0125～0.4μg/ml as the concentration range and / or 5￣30 min as incubation time under the abovecondition. It indicated that the assay time could be adjusted. These results would be important to the R&D of rapid detectioncard for assaying pesticide residue by rabbit serum AChE and improveing test accuracy.

In this paper , the extracting and purifying technology of the folacin in fruits and vegetables was studied. We designedthe orthogonal test for the solvent, developing agent and sorbent. The results showed: extracted with hot boiling metaphosphoricacid , absorbed with active carbon, developed with the mixture solution of 3% ammonia and 70% anhudrous alcohol, the conclusiongained the ends of extraction and purifying comparative completeness. And we measured the content of folacin with thefluorescence spectrophotometer. The recovery ratio was beyond 95%, the relative deviation was below 2.5%. The results wassatisfied.

Sulfite concentration is detected by low pressure ion chromatography (LPIC) with chemiluminescence due to thefact that hypoxanthine can sensitize the depression effect of sulfite on luminol- KMnO4 chemiluminescence reaction. The linerrange of sulfite concentration under optimal conditions is 5×10-8～4.8×10-6mol/L (r=0.9977)with a detection limit of 3.2×10-8mol/L, and RSD as 3.25% by assaying 5×10-7mol/L for five times. This simple, rapid and sensitive method is successfullyapplied to sulfite determination in food samples.

The olive kernel oils of Canarium album and Phyllanthus emblica were extracted with Soxhlet extraction method,and their fatty acid compositions were analyzed by GC/MS. The results showed that there were 13 fatty acids in Canarium albumkernel oil and 11 in Phyllanthus emblica kernel oil, the content of unsaturated fatty acid were 73.4% and 84.1%, linoleic acid 41.76% and 23.68%, oleic acid 30.48% and 58.37%, respectively. The odd number carbon acids (hexadecanoic acid, heptadecanoicacid) were determined out in the two oils. The two kernel oils have high nutritional and healthy value, and a wide developingpotency as new oil resources.

The technology has described the basic theory and implementation of assaying the major component density in cowmilk by using the combination of ultrasonic and electronic parameters. It has discussed the assaying methods of ultrasonic naturalparameter in cow milk especially. At the meantime, it has described the building methods of predictive model essential in the entireassay system. Finally, it has compared both ultrasonic and parametric methods. The result shows that ultrasonic method has gotsome advantages like stableness, correctness and quickness. The coefficient of variation for repeatability in assay is less than0.02 and the relative accuracy is less than 2%. It only takes 30 seconds to test each sample. It can fully meet the demands of practicalassay. The research provides a new method of no-wear fast assay for dairy products.

A method for the determination of acrylamide in food by gas chromatography was studied.The method consistedof extraction, bromization, evaporation and GC analysis. The GC analysis was carried out by using Elite-Wax capillary columnand electron capture detector. The linear range was 0～2000μg/kg and the limit of detection was 3μg/kg. The relative standarddeviation was 3.97%～7.48% and the recovery rate was 69.9%. The method has good stability and sensibility.

 There is red colour in some fruit and vegetable juice, in the case of regular titration to measure the VC content, soit is very difficult to judge titration terminal point. This experiment adopted the xylene and other organic agents to extract the coloringmatter and, then used the 2,6-dichlorindophenol to proceed titration. Through the verification, it shows satisfactory to results.

In Britton-Robinson buffer of pH2.36, the binding reaction of methyl blue with proteins enhances remarkably theresonance Rayleigh light-scattering (RLS) signal at maximum wavelength of 350 nm and forms complexes. It is found that theenhanced RLS intensities are in proportion to the concentrations of protein. Based on this, a new method for the determinationof proteins using methyl blue as indicator has been developed. Bovine serum albumin and human serum albumin could be determinedunder optimum conditions。The linear ranges of the calibration curves were 0～2.4mg/L for human serum albumin and 0～2.8mg/L for bovine serum albumin, and the detection limits were 39.3 and 43.4μg/L, respectively. The method had the advantagesof simplicity and high sensitivity. It has been used to determine total proteins in milk, soybean milk, human serum albumin andhuman urine. The results by this method are consistent with those obtained by the Coomassie brilliant blue G-250 assay。

SYBR Green I is widely used in real-time PCR applications as an intercalating dye. The use of melting curve analysiseliminates the necessity for agarose gel electrophoresis because the melting temperature (Tm) of the specific amplicon isanalogous to the detection of an electrophoretic band. In this paper we reported the development of a duplex real-time PCR suitablefor the detection of the bar and pat genes in genetically modifed organisms, based on the intercalating dye SYBR Green I and theanalysis of the melting curves of the amplified products. Using this method, different amplification products specific for the barand pat genes were obtained and identified by their specific Tm. We have combined amplification of these products in a numberof duplex reactions and showed the suitability of the methods for identification of GMOs with a sensitivity of 0.9% in duplexreactions.

 For understanding the acute toxicity of antistaling agent, kunming mice were divided into 24 groups which given fourkind antistaling agent at different dose. The result showed that the LD50 of antistaling agent A, B, C and D were 2889.117, 3237.07,3431.828 and 3546.835mg/kg. Antistaling agent C and D that only made of plant extracts have better safety and withoutantiseptic substance.

The antitumor activity and immune function of carotenoids from Potamogeton crispus L. (CEPC) were studied in thisarticle. Cyclophosphamide was the positive control and salad oil the negative control. H22-bearing mice were used as animalmodels. The changes of H22 tumor weight in vivo, the changes of MDA and SOD of liver in H22-bearing mice and the effect onimmune system such as DTH induced by DNFB, proliferation of lymphocytes and NK activity were investigated when differentdosage CEPC (5, 10, 20mg/kg·d) were used. It was shown that CEPC markedly inhibited tumor growth in the H22-bearing mice.It could also decrease MDA and increase SOD of liver in the H22-bearing mice. CEPC enhanced the immune function. So CEPChas both the antitumor effect and the immunity effect modulation.

This paper studied the effects of anthocyanin-rich extract from black rice on advanced plaques in ApoE deficientmice. ApoE deficient mice of 30 weeks of age were randomly divided into 3 groups: mice in two experimental groups were fedchow diets supplemented with black rice anthocyanin-rich extract and simvastatin seperately, while control group was fed anormal chow diet. Lipid profile of serum, plaque area and matrix metalloproteinase content in the innominate artery were assayed.Results showed that the anthocyanin-rich extract lowers TC, TG and LDL-C levels in serum, and decreases plaque area andMMP content in the innominate artery of mice. Conclusion: anthocyanin-rich extract can inhibit further development of advancedplaque in ApoE deficient mice.

The methods of hawthorn flavanoids extraction were studied. The results showed that the optimum conditions were :soaking solution 80% ethanol, temperature 80℃, the ratio of soild-to-liquid 1:10 and time 4 h. The extraction rate of flavonoidswas as high as 2.2%. It could reduce the hyperlipidemia of wistar TC and TG, also distinctly raise the HDL-C level. Soakingflavonoids of hawthorn could better adjust hyperlipidemia, and have the same effect as ZHIBITUO tablets.

 Objective: To observe the immune regulating effect of ginkgo albumin(GAP). Methods: In vivo normal mice, the effectson immune organ,carbongraular clearance ability, hemolysin production level, DTH induced by DNFB were assayed. MTTmethod was employed to investigate T lymphocyte and B lymphocyte proliferation LDH method to investigate the cytotoxic-ity of NK cells, the proliferation of thymus cells method to investigate activity of IL-2, and FACS method to investigate effectof GAP on T lymphocyte subsets. Results: In normal mice, GAP can increase spleen index and thymus index, improvephagocytosing function of peritoneal macrophage, significantly intensify DTH, and promote production of serum hemolysinsimmunized with SRBC. GAP can also promote proliferation of activated and inactivated T lymphocyte`as well as B lymphocyte,increase the cytotoxicity of NK cells and induce secretion of spleen lymphocyte. GAP can increase percent of L3T4+ cells(%),percent of Lyt2+ cells(%)and ratio of L3T4+ cells /Lyt2+ cells. Conclusion: GAP can significantly increase the immune regulatingfunction of normal mice.

Hot water dipping (HWD), cold shock (CS), salicylic acid (SA) and UV-C were used to induce postharvest diseaseresistance of banana fruits. The results indicated that HWD (53℃, 10min) significantly limited the enlargement of disease spots10 days after inoculation of Colletotrichum musae spores, meanwhile the softening and degreening of the fruits were delayed. Itwas also found that the effect of HWD on inducing resistance of banana fruits was related to the inoculating time and the harvestdate. SA treatment also limited the enlargement of disease spots to some extent, while CS accelerated the rotten rate, softeningand degreening of the banana fruits. Although UV-C (160￣320s) treatment delayed fruits softening, it led to larger disease spotsand severe brown peel compared to control fruits.

Bacteriostasis diameters of pumpkin extract’s influences on several test organisms were assayed by means of filter paperexamination. The effects on mutton preservation by pumpkin extract immersion combined with vacuum packaging during 0～4℃.The result showed that pumpkin extract had an evident bacteriostatic effect on the five test species and has effect on Saccharomycescerevisiae and Aspergillus, while no effect on Candida,penicillium and rhizopus. The results also showed that the storage timeof mutton soaked in pumpkin extract was 4～5 days longer than the control group soaked in water.

The paper studied the effects of various ingredients such as film-former, preservatives, moisture-retaining agent andanti-oxidant in the emulsion system on strawberry preservation by edible coatings. The results showed that the film formingmaterials used in the emulsion system were able to form continuous liquid film in the strawberry surface, with increased senseof shine of the product after drying. The low molecular weight chitosan showed good film-forming effect and ideal anti-bacteriumeffect as well. Sorbitol took an effect on controlling weight loss of strawberry at normal temperature. Vitamin C as anti-oxidanthad no obvious anti-browning effect during storage.

The effects of MAP (modified atmosphere package) on quality of minimally processed water bamboo shoot werestudied. The results showed that MAP with high density polyethylene film could maintain firmness and inhibit the toughnessof minimally processed water bamboo at the ambient temperature. Mathematical model was built to predict density of oxygenand carbondioxide in bags.

The unique absorption, high activity and diversity of polypeptides arouses people’s interest in application. It issignificant to study the separation and analyses of polypeptides so as to contribute to industrialization. High performance liquidchromatography(HPLC) is the effective and typical method of separating and analyzing polypeptides in laboratory andindustrial production because of its mass production advantage. This paper mainly introduced the application of the separationand analysis of polypeptides by HPLC.

 Pork is indispensable to our everyday diet which contains high-quality protein, and the quality of pork is influencedby many factors such as genotype, sex, age, nutrition, slaughtering, biochemistry change and carcass processing. Among these,nutrition and breeding are noticeable ones. In this paper, the studies on breeding aspect, energy level and feed nutrition, includingexogenous porcine somatotropin (PST), β-incitant, L-novain, lycine, Rucca Schidigera and electrolyte, which would affect thepork quality were also reviewed.

Acrylamide is a neurotoxin and a probable human carcinogen. It was reported that many heat-processed foods containedrelatively high levels of acrylamide. The paper reviewed the international research development of acrylamide toxicology, risk assessmentand its formation mechanism in food. Furthermore, some further research work was envisaged. So as to supply certain theory and methodreferences for studying acrylamide in Chinese foods, to control or reduce the acrylamide formation in food processing, to decrease theChinese diet exposure level of acrylamide and to promote the development of food safety and protect consumers health.

The paper reviewed the research advances of chemical compositions of sweet potato vines and leaves in recent years,especially in its bioactive components. Some advices were put forward for further research of sweet potato vines and leavesmeanwhile.

 Along with the flourishing study on the animal medicinal materials, it was proved that some endogenouspolysaccharides of animal organism had all kinds of bioactivities. The existence and distributing status of animal polysaccharidewere presented. The category and characteristics of animal polysaccharides were briefly reviewed. The methods of extracting,separating and determining the polysaccharide and its bioactivity and application were summarized especially.

The article introduced the development of health food in recent years and analyzed the factors that maybe inducedsafety problem in health food produced and used. It discussed the mechanism that cause disease and put forward relative settlemeasures.

Using the white gourd as the main raw material, a kind of lactic acid beverage with rich nutrition, equilibrium in nutrientsand retaining the flavor of walnuts was developed. After a long time of study, a main technological process and the optimumconditions of fermentation affecting the quality of products of the process were found.

In this article, the effects of enzymatic degration of chicken fat on flavor were studied through analyzing the flavorchanges after the degration, and the best conditions for the degration were found out. In addition, the effluences of the enzymedchicken fat as a participant in Maillard-reaction on the flavor of final products were compared with that of the unenzymed one.